PMID- 9062797
OWN - NLM
STAT- MEDLINE
DCOM- 19970603
LR  - 20191101
IS  - 0948-6143 (Print)
IS  - 0948-6143 (Linking)
VI  - 107
IP  - 2
DP  - 1997 Feb
TI  - Comparative FISH analysis of numerical chromosome 7 abnormalities in 5-micron and 
      15-micron paraffin-embedded tissue sections from prostatic carcinoma.
PG  - 121-6
AB  - Interphase fluorescence in situ hybridization (FISH) was performed on 
      15-micron-thick paraffin sections from prostatic carcinomas using a chromosome 
      7-specific alpha-satellite DNA probe. A confocal laser scanning microscope (CLSM) 
      was used for optical sectioning of the thick sections and reconstruction of 3D 
      images. The number of FISH signals was determined by a gallery of optical 
      sections evaluating only complete nuclei. To investiate the influence of section 
      thickness and truncation and nuclei on scoring results, we compared the FISH data 
      from 15-micron sections with signal counts obtained from 5-micron sections. The 
      latter were evaluated by conventional fluorescence microscopy in the same tumor 
      regions previously defined and marked on the slides. After statistical analysis 
      of spot frequencies in tumor and non-tumorous cells (chi 2 test), we transferred 
      the signal frequencies into a cytogenetic classification (-7, +7, polysomy 7). 
      Based on this classification, most cases showed more than one chromosome 7 
      aberration type. Trisomy 7 (+7) became apparent in 15-micron thick sections in 
      all 19 tumors, polysomy 7 (> 3 spots) in 18/19 cases, and monosomy 7 (-7) in 
      13/19 cases. In 5-micron sections, however, trisomy 7 and polysomy 7 were found 
      in only 7/19 and 13/19 cases, respectively, and monosomy 7 in 7/19 cases. When 
      comparing the classification results of tumor cells of the same tumor regions 
      originating either from 5-micron or 15-micron sections, the following 
      discrepancies were noted: in 15-micron sections exclusively, in 12/19 tumors, 
      trisomy 7 was found; in 6/19 cases, polysomy 7; in 8/19 cases, monosomy 7. The 
      high proportion of cases with tumor nuclei expressing only one hybridization 
      signal of chromosome 7 in 15-micron sections could be confirmed as monosomy 7 in 
      five selected cases by double-hybridization using centromere-specific probes for 
      chromosomes 7 and 12. These results demonstrate that numerical chromosome 7 
      aberrations are more frequently observed in thick (15-micron) paraffin-embedded 
      tissue sections by evaluating only complete nuclei. The use of routine sections 
      (5-micron) for interphase cytogenetic analyses is compromised by a remarkable 
      underestimation of the real chromosome copy numbers.
FAU - Aubele, M
AU  - Aubele M
AD  - GSF-National Research Center for Environment and Health, Institute of Pathology, 
      Oberschleissheim, Germany.
FAU - Zitzelsberger, H
AU  - Zitzelsberger H
FAU - Szucs, S
AU  - Szucs S
FAU - Werner, M
AU  - Werner M
FAU - Braselmann, H
AU  - Braselmann H
FAU - Hutzler, P
AU  - Hutzler P
FAU - Rodenacker, K
AU  - Rodenacker K
FAU - Lehmann, L
AU  - Lehmann L
FAU - Minkus, G
AU  - Minkus G
FAU - Hofler, H
AU  - Hofler H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Histochem Cell Biol
JT  - Histochemistry and cell biology
JID - 9506663
RN  - 0 (DNA Probes)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Adenocarcinoma/*pathology
MH  - Cell Nucleus/ultrastructure
MH  - Chromosome Aberrations/*pathology
MH  - Chromosome Disorders
MH  - Chromosomes, Human, Pair 7/*ultrastructure
MH  - DNA Probes
MH  - Fluorescein-5-isothiocyanate
MH  - Humans
MH  - In Situ Hybridization
MH  - Interphase
MH  - Male
MH  - Microscopy, Confocal
MH  - Microscopy, Fluorescence
MH  - Paraffin Embedding
MH  - Prostatic Neoplasms/*pathology
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - 10.1007/s004180050096 [doi]
PST - ppublish
SO  - Histochem Cell Biol. 1997 Feb;107(2):121-6. doi: 10.1007/s004180050096.