PMID- 9065649
OWN - NLM
STAT- MEDLINE
DCOM- 19970603
LR  - 20190914
IS  - 0937-4477 (Print)
IS  - 0937-4477 (Linking)
VI  - 254 Suppl 1
DP  - 1997
TI  - Detection of MET oncogene/hepatocyte growth factor receptor in lymph node 
      metastases from head and neck squamous cell carcinomas.
PG  - S138-43
AB  - The c-MET oncogene encodes the receptor for hepatocyte growth factor/scatter 
      factor (HGF/SF), which is known to stimulate the invasive growth of epithelial 
      cells cultured in vitro. The Met/HGF receptor is a heterodimeric transmembrane 
      tyrosine kinase, which is a prototype for a new family of growth factor 
      receptors. The c-MET oncogene is expressed in several types of epithelial tissue 
      including keratinocytes and is over-expressed in a number of human carcinomas. 
      Studies on various carcinoma cell lines have shown that over-expression and 
      structural alteration of the receptor result in its activation and confer 
      tumorigenesis. We have studied Met/HGF receptor expression in tissue specimens 
      from 34 patients with head and neck squamous cell carcinomas (HNSCC) and in 17 
      regional lymph node metastases. Western blot analysis was employed, using 
      monoclonal antibodies directed against either the intracellular or extracellular 
      domain of the receptor. Each sample was compared to its normal counterpart. The 
      receptor did not show any major structural alterations in HNSCC tissues, but its 
      expression was increased from 2- to 50-fold in about 70% of tumors. 
      Immunohistochemistry then showed that the same antibodies stained only a few 
      cells in the basal layer of normal squamous epithelium but intensely marked tumor 
      cells. In the lymph node metastases of Met-positive tumors, receptor expression 
      was maintained and sometimes increased with respect to primary tumors. 
      Immunohistochemical analysis of the metastatic lymph nodes showed that cells were 
      negative in the normal lymphatic tissue and strongly stained in tumor cells. 
      Over-expression of the Met/HGF receptor was found at all tumor stages but was 
      more significant in those associated with enlarged or multiple (N2-N3) lymph node 
      metastases. These data show that expression of the Met/HGF receptor may be 
      involved in the progression of HNSCC towards a metastatic phenotype and may be a 
      useful marker of head and neck tumor cell spread to regional lymph nodes.
FAU - Galeazzi, E
AU  - Galeazzi E
AD  - Department of Clinical Physiopathology, University of Turin School of Medicine, 
      Italy.
FAU - Olivero, M
AU  - Olivero M
FAU - Gervasio, F C
AU  - Gervasio FC
FAU - De Stefani, A
AU  - De Stefani A
FAU - Valente, G
AU  - Valente G
FAU - Comoglio, P M
AU  - Comoglio PM
FAU - Di Renzo, M F
AU  - Di Renzo MF
FAU - Cortesina, G
AU  - Cortesina G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur Arch Otorhinolaryngol
JT  - European archives of oto-rhino-laryngology : official journal of the European 
      Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the 
      German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
JID - 9002937
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Coloring Agents)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antibodies, Monoclonal
MH  - Biomarkers, Tumor/analysis
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/pathology/*secondary
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Coloring Agents
MH  - Disease Progression
MH  - Epithelium/drug effects/pathology
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Head and Neck Neoplasms/*pathology
MH  - Hepatocyte Growth Factor/analysis/genetics/pharmacology
MH  - Humans
MH  - Immunohistochemistry
MH  - Lymphatic Metastasis/*pathology
MH  - Lymphoid Tissue/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - Oncogenes/genetics
MH  - Phenotype
MH  - Protein-Tyrosine Kinases/analysis/genetics
MH  - Proto-Oncogene Proteins/*analysis/genetics/pharmacology
MH  - Proto-Oncogene Proteins c-met
MH  - Receptor Protein-Tyrosine Kinases/*analysis/genetics/pharmacology
MH  - Tumor Cells, Cultured
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1007/BF02439745 [doi]
PST - ppublish
SO  - Eur Arch Otorhinolaryngol. 1997;254 Suppl 1:S138-43. doi: 10.1007/BF02439745.