PMID- 9066027
OWN - NLM
STAT- MEDLINE
DCOM- 19970605
LR  - 20190728
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 15
IP  - 2
DP  - 1997 Feb
TI  - A quantitative study of the efficacy of a deletion mutant bovine herpesvirus-1 
      differential vaccine in reducing the establishment of latency by wildtype virus.
PG  - 123-8
AB  - Using quantitative polymerase chain reaction (PCR) we have studied the latency 
      established by wildtype (WT) bovine herpesvirus-1 (BHV-1) after challenge of 
      cattle that had been vaccinated with a double deletion (gC-/tk-) mutant BHV-1 
      vaccine. Fourteen animals were vaccinated intramuscularly with 2 ml containing 
      10(7.4) CCID50 (cell culture infectious dose 50%) of IBRV (NG) dltkdlgC and 
      challenged, along with six unvaccinated control animals, 30 days later with 
      10(8.2) CCID50 of WT BHV-1 (Cooper). The ability of this vaccine to prevent acute 
      clinical BHV-1 infection after this challenge has been previously reported. Sixty 
      days after challenge, eight of the vaccinates and the six control animals were 
      euthanitized and the trigeminal ganglia (TG) examined for the amount of WT BHV-1 
      DNA by an internal standard quantitative PCR. The quantitative protocol that we 
      used is based on co-amplification of BHV-1 gC specific sequences (present in WT 
      BHV-1 but absent in the vaccine strain) and sequences from the bovine growth 
      hormone (BGH) gene, which is used as an internal standard. The TG of the eight 
      vaccinates contained BHV-1 WT DNA, but in a statistically significantly lower 
      amount than the unvaccinated controls. These results are significant from the 
      standpoint that, to our knowledge, this is the first report of a systematic 
      quantitative approach to the study of the effect of BHV-1 vaccines on latency. 
      This technique could be used to measure and compare the efficiency of various 
      BHV-1 vaccines in preventing or diminishing latency, which is a significant 
      factor for the perpetuation of BHV-1 in cattle populations.
FAU - Galeota, J A
AU  - Galeota JA
AD  - Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln 
      68583-0905, USA.
FAU - Flores, E F
AU  - Flores EF
FAU - Kit, S
AU  - Kit S
FAU - Kit, M
AU  - Kit M
FAU - Osorio, F A
AU  - Osorio FA
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Vaccines, Inactivated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Ganglia/virology
MH  - *Gene Deletion
MH  - Herpesviridae Infections/genetics/*prevention & control/*veterinary
MH  - Herpesvirus 1, Bovine/*genetics/*immunology/physiology
MH  - Mutation/*immunology
MH  - Trigeminal Nerve/virology
MH  - Vaccines, Inactivated/genetics/immunology
MH  - Viral Vaccines/genetics/*immunology
MH  - Virus Latency/genetics/*immunology
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - S0264-410X(96)00165-X [pii]
AID - 10.1016/s0264-410x(96)00165-x [doi]
PST - ppublish
SO  - Vaccine. 1997 Feb;15(2):123-8. doi: 10.1016/s0264-410x(96)00165-x.