PMID- 9066120
OWN - NLM
STAT- MEDLINE
DCOM- 19970411
LR  - 20191024
IS  - 0965-1748 (Print)
IS  - 0965-1748 (Linking)
VI  - 27
IP  - 2
DP  - 1997 Feb
TI  - A single amino acid change in the para sodium channel protein is associated with 
      knockdown-resistance (kdr) to pyrethroid insecticides in German cockroach.
PG  - 93-100
AB  - Previous genetic and pharmacological studies suggest that knockdown-resistance 
      (kdr) to pyrethroid insecticides likely results from a mutation(s) in the para 
      sodium channel gene. In this study, para sodium channel genes from an 
      insecticide-susceptible German cockroach strain, CSMA, and a kdr-type German 
      cockroach strain, Ectiban-R, were cloned and sequenced. Comparison of the 
      nucleotide sequences of paraCSMA and paraEctiban-R revealed two nucleotide 
      differences at nt 1491 and nt 2979, respectively. Only the difference at nt 2979 
      (G in paraCSMA and C in paraEctiban-R) resulted in an amino acid change (Leu993 
      in CSMA and Phe993 in Ectiban-R). Leu993/Phe993 is located in the highly 
      conserved membrane-spanning segment 6 of domain II (IIS6). Furthermore, reverse 
      transcription-polymerase chain reaction (RT-PCR) sequence analysis using another 
      two insecticide-susceptible and one kdr German cockroach strain, revealed that 
      C2979 was specifically associated with kdr resistant strains, whereas only G2979 
      was present in all susceptible strains. These results suggest that a single amino 
      acid change from Leu993 to Phe993 is responsible for the kdr-type resistance in 
      German cockroach.
FAU - Dong, K
AU  - Dong K
AD  - Department of Entomology, Michigan State University, East Lansing 48824, USA.
LA  - eng
SI  - GENBANK/U73583
SI  - GENBANK/U73584
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Insect Biochem Mol Biol
JT  - Insect biochemistry and molecular biology
JID - 9207282
RN  - 0 (DNA, Complementary)
RN  - 0 (Insect Proteins)
RN  - 0 (Pyrethrins)
RN  - 0 (Sodium Channels)
RN  - 47E5O17Y3R (Phenylalanine)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - *Cockroaches/genetics
MH  - DNA, Complementary
MH  - Insect Proteins/*genetics/metabolism
MH  - *Insecticide Resistance
MH  - Leucine
MH  - Molecular Sequence Data
MH  - Phenylalanine
MH  - *Pyrethrins
MH  - Sequence Homology, Amino Acid
MH  - Sodium Channels/*genetics/metabolism
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - S0965174896000823 [pii]
AID - 10.1016/s0965-1748(96)00082-3 [doi]
PST - ppublish
SO  - Insect Biochem Mol Biol. 1997 Feb;27(2):93-100. doi: 
      10.1016/s0965-1748(96)00082-3.