PMID- 9066984
OWN - NLM
STAT- MEDLINE
DCOM- 19970703
LR  - 20191101
IS  - 1077-3150 (Print)
IS  - 1077-3150 (Linking)
VI  - 60
IP  - 1
DP  - 1997 Feb
TI  - Exclusion of the nuclear factor-kappa B3 (REL A) gene as candidate for the 
      multiple endocrine neoplasia type 1 (MEN 1) gene.
PG  - 76-9
AB  - Multiple endocrine neoplasia type 1 (MEN 1) is inherited as an autosomal dominant 
      disorder, characterized by neoplasia and hyperplasia in specific endocrine 
      organs. The MEN 1 gene, which is most probably a tumor suppressor gene, has been 
      localized to a region of approximately 900 kb on chromosome 11q13. The nuclear 
      factor-kappa B (NF-kappa B) is a transcription factor with pleiotropic 
      expression, which is involved in the regulation of expression of many cellular 
      genes. The p50/p65 heterodimer is the most abundant form of NF-kappa B. The gene 
      encoding the p65 subunit (NF-kappa B3/REL A) was recently localized in the 900-kb 
      MEN 1 region and was considered a good candidate gene for MEN 1. The structure 
      and nucleotide sequence of the NF-kappa B3 coding region in MEN 1 patients were 
      compared with those of non-MEN 1 subjects, to determine the potential role of 
      this gene in MEN 1 tumorigenesis. Southern blot analysis with constitutional DNA 
      from probands of 14 independent MEN 1 families and DNA from four MEN 1 tumor 
      specimens did not reveal any structural abnormality of the NF-kappa B3 gene. 
      Direct sequencing of cDNAs from two affected subjects from 2 different MEN 1 
      families, as well as nucleotide sequence analysis of exon/intron boundaries in 
      these patients, did not reveal MEN 1-specific point mutations or other small 
      structural aberrations in the NF-kappa B3 gene. These results make it very 
      unlikely that the NF-kappa B3 gene is the gene responsible for the development of 
      MEN 1.
FAU - Landsvater, R M
AU  - Landsvater RM
AD  - Department of Internal Medicine, University Hospital Utrecht, The Netherlands.
FAU - de Wit, M J
AU  - de Wit MJ
FAU - Peterson, L F
AU  - Peterson LF
FAU - Sinke, R J
AU  - Sinke RJ
FAU - Geurts van Kessel, A
AU  - Geurts van Kessel A
FAU - Lips, C J
AU  - Lips CJ
FAU - Hoppener, J W
AU  - Hoppener JW
LA  - eng
SI  - GENBANK/Z22948
SI  - GENBANK/Z22949
SI  - GENBANK/Z22950
SI  - GENBANK/Z22951
SI  - GENBANK/Z22952
SI  - GENBANK/Z22953
SI  - GENBANK/Z22954
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Mol Med
JT  - Biochemical and molecular medicine
JID - 9508702
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (NF-kappa B)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factor RelA)
SB  - IM
MH  - Base Sequence
MH  - DNA, Complementary
MH  - DNA, Neoplasm/*analysis
MH  - Humans
MH  - Molecular Sequence Data
MH  - Multiple Endocrine Neoplasia Type 1/*genetics
MH  - NF-kappa B/*genetics
MH  - Nuclear Proteins/*genetics
MH  - Transcription Factor RelA
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - S1077315096925612 [pii]
AID - 10.1006/bmme.1996.2561 [doi]
PST - ppublish
SO  - Biochem Mol Med. 1997 Feb;60(1):76-9. doi: 10.1006/bmme.1996.2561.