PMID- 9067436
OWN - NLM
STAT- MEDLINE
DCOM- 19970417
LR  - 20190607
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 75
IP  - 4
DP  - 1996
TI  - A novel multicolor hybridization scheme applied to localization of a transcribed 
      sequence (D10S170/H4) and deletion mapping in the thyroid cancer cell line TPC-1.
PG  - 254-7
AB  - The sequence-tagged site (STS) D10S170, also referred to as H4, is a gene of 
      unknown function. Its 5' end was found fused to the catalytic domain of the RET 
      protooncogene to generate RET/PTC 1, the most common form of PTC oncogenes in 
      human papillary thyroid carcinoma. This gene has previously been assigned to a 
      very large genomic region, 10q11.22-->q22.1. Here, we describe the application of 
      a novel hybridization scheme to the physical and genetic mapping of D10S170. 
      First, we selected a homologous large-insert DNA clone from a human P1 library by 
      filter hybridization and confirmed its authenticity by Southern blot analysis. 
      Triple-color fluorescence in situ hybridization (FISH) experiments mapped this 
      clone to l0q21.2-->q21.3. "Binning" experiments were performed using a 
      quadruple-color FISH approach aimed toward placing the gene in a genetic interval 
      defined by differentially labeled P1 DNA probes containing known polymorphic 
      markers. We found that multicolor FISH greatly expedites chromosomal mapping. 
      Finally, we applied our FISH approach to determine the extent of deletion 
      involving this locus (D10S170) in a papillary thyroid cancer cell line, TPC-1.
FAU - Jossart, G H
AU  - Jossart GH
AD  - Department of Surgery, University of California San Francisco/Mount Zion 
      Hospital, USA.
FAU - O'Brien, B
AU  - O'Brien B
FAU - Cheng, J F
AU  - Cheng JF
FAU - Tong, Q
AU  - Tong Q
FAU - Jhiang, S M
AU  - Jhiang SM
FAU - Duh, Q
AU  - Duh Q
FAU - Clark, O H
AU  - Clark OH
FAU - Weier, H U
AU  - Weier HU
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Genetic Markers)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Proto-Oncogene Proteins)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-ret)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Ret protein, Drosophila)
RN  - EC 2.7.10.1 (ret-PTC fusion oncoproteins, human)
SB  - IM
MH  - Carcinoma, Papillary/*genetics/pathology
MH  - Chromosome Inversion
MH  - Chromosome Mapping/*methods
MH  - Chromosomes, Human, Pair 10/*genetics/ultrastructure
MH  - Chromosomes, Human, Pair 21/genetics/ultrastructure
MH  - DNA Probes
MH  - DNA, Neoplasm/genetics
MH  - *Drosophila Proteins
MH  - *Fluorescent Dyes
MH  - *Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Protein-Tyrosine Kinases
MH  - Proto-Oncogene Proteins/genetics
MH  - Proto-Oncogene Proteins c-ret
MH  - Receptor Protein-Tyrosine Kinases/genetics
MH  - *Sequence Deletion
MH  - Thyroid Neoplasms/*genetics/pathology
MH  - Translocation, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1159/000134495 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1996;75(4):254-7. doi: 10.1159/000134495.