PMID- 9067437
OWN - NLM
STAT- MEDLINE
DCOM- 19970417
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 75
IP  - 4
DP  - 1996
TI  - Horseradish peroxidase-labeled oligonucleotides and fluorescent tyramides for 
      rapid detection of chromosome-specific repeat sequences.
PG  - 258-62
AB  - We present a sensitive and rapid fluorescence in situ hybridization (FISH) 
      strategy for detecting chromosome-specific repeat sequences. It uses horseradish 
      peroxidase (HRP)-labeled oligonucleotide sequences in combination with 
      fluorescent tyramide-based detection. After in situ hybridization, the HRP 
      conjugated to the oligonucleotide probe is used to deposit fluorescently labeled 
      tyramide molecules at the site of hybridization. The method features full 
      chemical synthesis of probes, strong FISH signals, and short processing periods, 
      as well as multicolor capabilities.
FAU - van Gijlswijk, R P
AU  - van Gijlswijk RP
AD  - Department of Cytochemistry and Cytometry, Leiden University, The Netherlands.
FAU - Wiegant, J
AU  - Wiegant J
FAU - Vervenne, R
AU  - Vervenne R
FAU - Lasan, R
AU  - Lasan R
FAU - Tanke, H J
AU  - Tanke HJ
FAU - Raap, A K
AU  - Raap AK
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (TSA-Direct BlueFISH)
RN  - 0 (TSA-Direct GreenFISH)
RN  - 0 (TSA-Direct RedFISH)
RN  - 42HK56048U (Tyrosine)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
SB  - IM
MH  - Chromosome Mapping/*methods
MH  - Chromosomes, Human/genetics/*ultrastructure
MH  - *Fluorescent Dyes
MH  - *Horseradish Peroxidase
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphocytes/ultrastructure
MH  - Male
MH  - *Oligonucleotide Probes
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Sensitivity and Specificity
MH  - Tyrosine/*analogs & derivatives
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1159/000134496 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1996;75(4):258-62. doi: 10.1159/000134496.