PMID- 9067586
OWN - NLM
STAT- MEDLINE
DCOM- 19970404
LR  - 20190914
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 11
IP  - 3
DP  - 1997 Mar
TI  - Application of fluorescence in situ hybridization to detect residual leukemic 
      cells with 9;22 and 15;17 translocations.
PG  - 436-40
AB  - We performed fluorescence in situ hybridization (FISH) upon 9;22 and 15;17 
      translocation-positive bone marrow cells to monitor the clinical course of 46 
      patients with chronic myelocytic leukemia (CML) and nine with acute promyelocytic 
      leukemia (AML M3) who received chemotherapy and/or bone marrow transplantation 
      (BMT). M-BCR-ABL and PML-RAR alpha probes were used to detect translocations of 
      t(9;22) and t(15;17), respectively. Signals from CML patients treated with 
      interferon (17 patients) or BMT (29 patients) were 0.5-15% positive for the 9;22 
      translocation. Among nine M3 patients who received extensive chemotherapy or BMT, 
      1-5% were positive for the 15;17 translocation. A highly sensitive FISH procedure 
      using both translocation probes and a whole chromosome Y probe was established 
      and applied to eight sex-mismatched BMT patients (seven CML and one AML M3), in 
      which 0.1-0.6% of signals positive for the specific translocations were detected. 
      These results suggested that interphase FISH is powerful enough to identify minor 
      cell populations of 9;22 or 15;17 translocations after therapy, as well as to 
      detect specific chromosome abnormalities at diagnosis.
FAU - Tanaka, K
AU  - Tanaka K
AD  - Department of Cancer Cytogenetics, Research Institute for Radiation Biology and 
      Medicine, Hiroshima University, Minami-ku, Japan.
FAU - Arif, M
AU  - Arif M
FAU - Eguchi, M
AU  - Eguchi M
FAU - Kumaravel, T S
AU  - Kumaravel TS
FAU - Ueda, R
AU  - Ueda R
FAU - Ohno, R
AU  - Ohno R
FAU - Iwato, K
AU  - Iwato K
FAU - Kyo, T
AU  - Kyo T
FAU - Dohy, H
AU  - Dohy H
FAU - Kamada, N
AU  - Kamada N
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (DNA Probes)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Bone Marrow/ultrastructure
MH  - Chromosomes, Human, Pair 15
MH  - Chromosomes, Human, Pair 17
MH  - Chromosomes, Human, Pair 22
MH  - Chromosomes, Human, Pair 9
MH  - DNA Probes
MH  - Fusion Proteins, bcr-abl/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/diagnosis/*genetics
MH  - Leukemia, Promyelocytic, Acute/diagnosis/*genetics
MH  - Microscopy, Confocal
MH  - Neoplasm, Residual
MH  - Neutrophils/ultrastructure
MH  - *Translocation, Genetic
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - 10.1038/sj.leu.2400583 [doi]
PST - ppublish
SO  - Leukemia. 1997 Mar;11(3):436-40. doi: 10.1038/sj.leu.2400583.