PMID- 9067844
OWN - NLM
STAT- MEDLINE
DCOM- 19970606
LR  - 20231213
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 379
IP  - 4
DP  - 1997 Mar 24
TI  - Connexin32 in oligodendrocytes and association with myelinated fibers in mouse 
      and rat brain.
PG  - 571-91
AB  - The distribution and cellular localization of connexin32 (Cx32) in the brain and 
      spinal cord of the mouse and rat was investigated by light microscope (LM) and 
      electron microscope (EM) immunohistochemistry by using several different 
      antibodies against Cx32. By double immunofluorescence staining for Cx32 and 
      either the oligodendrocyte markers cyclic nucleotide phosphodiesterase (CNPase) 
      or Rip, Cx32 was consistently found in oligodendrocyte cell bodies and proximal 
      processes. Cx32 immunoreactivity was also clearly visualized along CNPase- and 
      Rip-positive myelinated fibers. Both immunopositive cells and fibers were 
      heterogeneously distributed and were often more intensely labeled when dispersed 
      in or associated with regions of gray matter than when concentrated in major 
      white matter tracts. Labeling of myelin sheaths along fibers was restricted to 
      subpopulations of myelinated axons. In the cerebellar cortex, for example, it was 
      selectively localized to sheaths around Purkinje cell axons. Punctate staining, 
      distinct from that corresponding to cells or fibers, was evident in the olfactory 
      bulb and hippocampus. By EM, oligodendrocytes exhibited cytoplasmic labeling 
      associated with rough endoplasmic reticulum and Golgi apparatus. Their processes 
      were intermittently stained, most intensely when surrounding myelinated fibers 
      and occasionally in paranodal loops. Cx32-immunoreactive gap junctions with 
      symmetric labeling (staining on both junctional membranes) were observed between 
      oligodendrocytic somata and processes as well as between presumptive 
      oligodendrocytic processes. Unidentifiable elements forming asymmetrically 
      labeled gap junctions (staining only one side of junctional membranes) were less 
      frequently encountered. Western blot analysis confirmed anti-Cx32 antibody 
      detection of Cx32 in whole brain homogenates and an enrichment of the protein in 
      isolated myelin fractions. These results are consistent with earlier 
      ultrastructural studies showing the occurrence of inter-oligodendrocytic gap 
      junctions, but indicate that these may be more prevalent than previously thought. 
      Furthermore, the results suggest a specialized role of gap junctions composed of 
      Cx32 along myelinated fibers belonging to subpopulations of neurons.
FAU - Li, J
AU  - Li J
AD  - Department of Physiology, University of Manitoba, Winnipeg, Canada.
FAU - Hertzberg, E L
AU  - Hertzberg EL
FAU - Nagy, J I
AU  - Nagy JI
LA  - eng
GR  - P01AG02126/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Connexins)
RN  - 0 (Nerve Tissue Proteins)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain Chemistry/*physiology
MH  - *Brain Mapping
MH  - Connexins/*analysis
MH  - Immunoenzyme Techniques
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Microscopy, Electron
MH  - Nerve Fibers, Myelinated/*chemistry
MH  - Nerve Tissue Proteins/*analysis
MH  - Oligodendroglia/*chemistry
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord/chemistry
MH  - Gap Junction beta-1 Protein
EDAT- 1997/03/24 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/03/24 00:00
PHST- 1997/03/24 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/03/24 00:00 [entrez]
AID - 10.1002/(SICI)1096-9861(19970324)379:4<571::AID-CNE8>3.0.CO;2-# [pii]
AID - 10.1002/(sici)1096-9861(19970324)379:4<571::aid-cne8>3.0.co;2-# [doi]
PST - ppublish
SO  - J Comp Neurol. 1997 Mar 24;379(4):571-91. doi: 
      10.1002/(sici)1096-9861(19970324)379:4<571::aid-cne8>3.0.co;2-#.