PMID- 9068276
OWN - NLM
STAT- MEDLINE
DCOM- 19970623
LR  - 20190717
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 69
IP  - 5
DP  - 1997 Mar 1
TI  - DNA analysis using an electrospray scanning mobility particle sizer.
PG  - 919-25
AB  - A scanning mobility particle sizer (SMPS) allows size separation of gas phase 
      particles according to their electrophoretic mobilities. The addition of an 
      electrospray source (ES) recently allowed extension of SMPS analysis to the 
      macromolecular range. We demonstrate here the application of ES-SMPS to nucleic 
      acids analysis. Single- and double-stranded DNA molecules ranging from 6.1 kDa 
      (single-stranded DNA 20 nucleotides in length) to 300 kDa (500 base-pair 
      double-stranded DNA) were separated and detected by ES-SMPS at the picomole to 
      femtomole levels. The measured electrophoretic mobility diameters were found to 
      correlate with the analytes' molecular weights, while the peak areas could yield 
      quantitative information. No fragmentation of DNA was observed under the 
      conditions employed. Different apparent densities were observed for 
      single-stranded and double-stranded DNAs, showing a different behavior for each 
      type of biomolecule. The total analysis time was about 3 min/spectrum. Further 
      optimization of ES-SMPS is expected to make it a fast and sensitive technique for 
      biopolymer characterization.
FAU - Mouradian, S
AU  - Mouradian S
AD  - Department of Chemistry, University of Wisconsin, Madison 53706-1396, USA.
FAU - Skogen, J W
AU  - Skogen JW
FAU - Dorman, F D
AU  - Dorman FD
FAU - Zarrin, F
AU  - Zarrin F
FAU - Kaufman, S L
AU  - Kaufman SL
FAU - Smith, L M
AU  - Smith LM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Oligonucleotides)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/*chemistry
MH  - DNA Fragmentation
MH  - Electrophoresis
MH  - Hydrogen-Ion Concentration
MH  - Oligonucleotides/chemistry
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - 10.1021/ac960785k [doi]
PST - ppublish
SO  - Anal Chem. 1997 Mar 1;69(5):919-25. doi: 10.1021/ac960785k.