PMID- 9068948
OWN - NLM
STAT- MEDLINE
DCOM- 19970527
LR  - 20190831
IS  - 0271-3683 (Print)
IS  - 0271-3683 (Linking)
VI  - 16
IP  - 2
DP  - 1997 Feb
TI  - Calcium signals from intact rabbit ciliary epithelium observed with confocal 
      microscopy.
PG  - 166-75
AB  - PURPOSE: We investigated patterns of evoked calcium signals to learn about the 
      function of the calcium second messenger system in ciliary epithelium. METHODS: 
      Isolated infact ciliary processes were loaded with fluo-3/AM and observed with a 
      Bio-Rad MRC-600 laser scanning confocal imaging system, before, during, and after 
      perfusion with catecholamines, cholinergic agents, and autocoids. RESULTS: One 
      microM acetylcholine (ACH) and 10 microM carbachol (CARB) induced an 
      atropine-sensitive increase in intracellular free calcium ion concentration 
      ([Ca2+]i), considerably greater in NPE than in PE. 10 microM epinephrine (EPI) 
      and 100 microM phenylephrine (PHE) increased [Ca2+]i in NPE and PE, in this case 
      PE > NPE. These effects were blocked by prazosin. 10 mM caffeine (CAF) increased 
      of [Ca2+]i in NPE and PE (NPE > PE) and sometimes produced very slow oscillations 
      with an interval of 10 to 25 s. Prior administration of CAF strongly suppressed 
      the effects of ACH, CARB, EPI, PHE, histamine, and adenosine 5-triphosphate 
      (ATP). One hundred microM ryanodine (RYA) or thapsigargin (TG) increased [Ca2+]i 
      in NPE and PE (NPE > PE). CONCLUSIONS: In the ciliary epithelium: (1) different 
      patterns of evoked transients and oscillations of calcium were seen in response 
      to agonists; (2) NPE appeared to contain a predominance of muscarinic receptors, 
      while the PE is dominated by alpha 1-adrenergic receptors and (3) the increase in 
      [Ca2+]i by CAF or RYA or TG in either cell layer and the blocking effects of 
      these agents upon agonist, evoking increases in [Ca2+]i, suggested involvement of 
      both the cyclic adenosine diphosphate ribose and the inositol 1, 4, 5 
      triphosphate (InsP3) systems in the regulation of intracellular calcium.
FAU - Suzuki, Y
AU  - Suzuki Y
AD  - Department of Ophthalmology and Visual Science, Yale University School of 
      Medicine, New Haven, CT 06520-8061, USA.
FAU - Nakano, T
AU  - Nakano T
FAU - Sears, M
AU  - Sears M
LA  - eng
GR  - EY-00785-24/EY/NEI NIH HHS/United States
GR  - EY-08879-06/EY/NEI NIH HHS/United States
GR  - P30DK34989/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Aniline Compounds)
RN  - 0 (Catecholamines)
RN  - 0 (Cholinergic Agents)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Xanthenes)
RN  - 23D4W0B50Y (Fluo-3)
RN  - 3G6A5W338E (Caffeine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Aniline Compounds
MH  - Animals
MH  - Caffeine/pharmacology
MH  - Calcium/*metabolism
MH  - Catecholamines/pharmacology
MH  - Cholinergic Agents/pharmacology
MH  - Ciliary Body/cytology/*metabolism
MH  - Epithelial Cells
MH  - Epithelium/metabolism
MH  - Fluorescent Dyes
MH  - In Vitro Techniques
MH  - Male
MH  - Microscopy, Confocal
MH  - Rabbits
MH  - *Signal Transduction
MH  - Xanthenes
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - 10.1076/ceyr.16.2.166.5095 [doi]
PST - ppublish
SO  - Curr Eye Res. 1997 Feb;16(2):166-75. doi: 10.1076/ceyr.16.2.166.5095.