PMID- 9069120
OWN - NLM
STAT- MEDLINE
DCOM- 19970619
LR  - 20230320
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 8
IP  - 3
DP  - 1997 Mar
TI  - Mapping of microsatellite markers developed from a flow-sorted swine chromosome 6 
      library.
PG  - 193-9
AB  - Swine Chromosome (Chr) 6-enriched libraries, generated with size-fractionated DNA 
      isolated from chromosomes sorted by flow cytometry, have been used to develop new 
      Chr 6 microsatellite markers. Chromosome isolation procedures were established to 
      reproducibly prepare high quality chromosomes from phytohemagglutinin 
      (PHA)-stimulated swine peripheral blood lymphocytes and to sort individual 
      chromosomes after staining with Hoechst 33258 and chromomycin A3. Chromosome 
      purity was verified by specific staining of swine Chr 6 with fluorescence in situ 
      hybridization (FISH) by use of painting probes generated by degenerate 
      oligonucleotide-primed polymerase chain reaction (DOP-PCR) amplification of as 
      few as 300 sorted Chr 6. For library construction, DNA was extracted from 
      flow-sorted pools representing Chr 6, amplified, size selected for fragments from 
      300 to 700 bp, and ligated into pBluescript SK II+ or Lambda ZAP Express. The 
      libraries were then screened with a radiolabeled poly-(dCA) DNA probe. Of 107 
      (CA)n repeat-containing clones verified by sequencing, 21 were polymorphic and 
      used to genotype the University of Illinois swine reference families. Linkage 
      analysis was then performed with CRIMAP 2.4 (LOD > 3.0), and the results showed 
      that 15 of the microsatellites mapped to swine Chr 6. At least three of these new 
      markers map to locations where there were gaps in the consensus Chr 6 map. 
      Another four markers, because of their PIC values, should provide more 
      informative markers in other areas of the map. Most of the new markers can also 
      be used for automated genotyping with fluorescent labeling. This set of 15 new 
      Chr 6 markers will, therefore, be useful in helping to define QTL associated with 
      swine Chr 6.
FAU - Grimm, D R
AU  - Grimm DR
AD  - USDA-ARS-IDRL, BARC-East, Building 1040, Room 105, Beltsville, MD 20705, USA.
FAU - Goldman, T
AU  - Goldman T
FAU - Holley-Shanks, R
AU  - Holley-Shanks R
FAU - Buoen, L
AU  - Buoen L
FAU - Mendiola, J
AU  - Mendiola J
FAU - Schook, L B
AU  - Schook LB
FAU - Louis, C
AU  - Louis C
FAU - Rohrer, G A
AU  - Rohrer GA
FAU - Lunney, J K
AU  - Lunney JK
LA  - eng
SI  - GENBANK/U24283
SI  - GENBANK/U24284
SI  - GENBANK/U24285
SI  - GENBANK/U24286
SI  - GENBANK/U62944
SI  - GENBANK/U62945
SI  - GENBANK/U62946
SI  - GENBANK/U62947
SI  - GENBANK/U62948
SI  - GENBANK/U62949
SI  - GENBANK/U62950
SI  - GENBANK/U62951
SI  - GENBANK/U62952
SI  - GENBANK/U62953
SI  - GENBANK/U62954
SI  - GENBANK/U62955
SI  - GENBANK/U62956
SI  - GENBANK/U62957
SI  - GENBANK/U62958
SI  - GENBANK/U62959
SI  - GENBANK/U66700
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
SB  - IM
MH  - Animals
MH  - *Chromosome Mapping
MH  - Female
MH  - Flow Cytometry
MH  - Genetic Linkage
MH  - Genomic Library
MH  - Genotype
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - *Microsatellite Repeats
MH  - Molecular Sequence Data
MH  - Swine
MH  - Swine, Miniature/*genetics
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - 10.1007/s003359900388 [doi]
PST - ppublish
SO  - Mamm Genome. 1997 Mar;8(3):193-9. doi: 10.1007/s003359900388.