PMID- 9071072
OWN - NLM
STAT- MEDLINE
DCOM- 19970409
LR  - 20061115
IS  - 0125-2208 (Print)
IS  - 0125-2208 (Linking)
VI  - 79 Suppl 1
DP  - 1996 Dec
TI  - Determination of the ratio of X and Y bearing spermatozoa after albumin gradient 
      method using double-labelled fluorescence in situ hybridization (FISH).
PG  - S88-95
AB  - Determination of X and Y human spermatozoa by double labelled fluorescence in 
      situ hybridization (FISH) is a new and more reliable method than Quinacrine 
      hydrochloride staining for Y sperm identification. A preliminary report on 
      determination of the ratio of X and Y sperm of normal semen of fifteen donors by 
      double labelled FISH method before and after Y sperm separation by Ericsson 
      albumin column method. This is currently popularized in Thailand. Results of 
      standardized FISH technique has an average success rate of approximately 95 
      percent. Results of the experiment showed that the ratio of X:Y sperm before and 
      after albumin column filtration is 50.03:49.80 and 48.77:51.00, respectively. Few 
      spermatozoa have double sex chromosomes, XX, XY or YY. In conclusion, Y sperm 
      selection by Ericsson's method is ineffective for male sex preselection.
FAU - Aribarg, A
AU  - Aribarg A
AD  - Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn 
      University, Bangkok, Thailand.
FAU - Ngeamvijawat, J
AU  - Ngeamvijawat J
FAU - Chanprasit, Y
AU  - Chanprasit Y
FAU - Sukcharoen, N
AU  - Sukcharoen N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Thailand
TA  - J Med Assoc Thai
JT  - Journal of the Medical Association of Thailand = Chotmaihet thangphaet
JID - 7507216
RN  - 0 (Serum Albumin)
SB  - IM
MH  - Cell Separation
MH  - Centrifugation, Density Gradient
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Serum Albumin
MH  - Spermatozoa/*ultrastructure
MH  - *X Chromosome
MH  - *Y Chromosome
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
PST - ppublish
SO  - J Med Assoc Thai. 1996 Dec;79 Suppl 1:S88-95.