PMID- 9071573
OWN - NLM
STAT- MEDLINE
DCOM- 19970612
LR  - 20061115
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 18
IP  - 3
DP  - 1997 Mar
TI  - A 3p21.3 region is preferentially eliminated from human chromosome 3/mouse 
      microcell hybrids during tumor growth in SCID mice.
PG  - 200-11
AB  - We have previously shown that four markers spanning the 3p24-p21.3 region, THRB, 
      AP20R, D3S1029, and D3S32, were regularly eliminated from three human chromosome 
      3 (chr3)/mouse microcell hybrids (MCHs) during tumor growth in SCID mice. In an 
      attempt to narrow down the eliminated region, we have studied 22 new SCID mouse 
      tumors derived from 5 MCH lines carrying human chr3. They were analyzed by 
      fluorescence in situ hybridization (FISH), Southern blotting, and polymerase 
      chain reaction (PCR). MCHs that carried human chr1, chr8, chr13, and chr17 were 
      examined as controls. We could identify a common eliminated region (CER) at 
      3p21.3, bordered distally by D3S1260 and proximally by D3S643/D3F15S2. Eight of 
      53 chr3-specific PCR markers, AP20R, D3S966, D3S3559, D3S1029, WI-7947, D3S2354, 
      AFMb362wb9, and D3S32. were localized within the CER. This finding is consistent 
      with the notion that a tumor suppressor gene may be located in this area, as 
      suggested by frequent loss of heterozygosity (LOH) within this region observed in 
      several types of solid tumors.
FAU - Kholodnyuk, I
AU  - Kholodnyuk I
AD  - Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden.
FAU - Kost-Alimova, M
AU  - Kost-Alimova M
FAU - Kashuba, V
AU  - Kashuba V
FAU - Gizatulin, R
AU  - Gizatulin R
FAU - Szeles, A
AU  - Szeles A
FAU - Stanbridge, E J
AU  - Stanbridge EJ
FAU - Zabarovsky, E R
AU  - Zabarovsky ER
FAU - Klein, G
AU  - Klein G
FAU - Imreh, S
AU  - Imreh S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Animals
MH  - Blotting, Southern
MH  - *Chromosome Aberrations
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 3/*genetics
MH  - DNA, Neoplasm/analysis
MH  - Fibrosarcoma/*genetics
MH  - *Genes, Tumor Suppressor
MH  - Genetic Markers
MH  - Heterozygote
MH  - Humans
MH  - Hybrid Cells/*ultrastructure
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Mice, SCID
MH  - Polymerase Chain Reaction
MH  - Sequence Deletion
MH  - Translocation, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1997/03/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - 10.1002/(SICI)1098-2264(199703)18:3<200::AID-GCC6>3.0.CO;2-5 [pii]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1997 Mar;18(3):200-11.