PMID- 9071576
OWN - NLM
STAT- MEDLINE
DCOM- 19970612
LR  - 20111117
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 18
IP  - 3
DP  - 1997 Mar
TI  - EWS-ERG fusion transcript produced by chromosomal insertion in a Ewing sarcoma.
PG  - 228-31
AB  - The EWS gene is fused in Ewing sarcoma-like tumors by a chromosomal translocation 
      to one of the four ETS-family genes: FLI1, ERG, ETV1, and E1AF. The orientation 
      of EWS and FLI1 on chromosomes 22 and 11, respectively, is 5' centromeric and 3' 
      telomeric, whereas that of ERG on chromosome 21 is the reverse. Although 10% of 
      Ewing-family tumors express the EWS-ERG fusion transcript, there have been no 
      reports on tumors with t(21;22)(q22;q12) identified by banding cytogenetics. We 
      found the karyotype 50, XY, +8, +8, +12, +mar in all metaphase cells from a 
      tumor. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis 
      performed on the tumor and direct sequencing of the products identified the 
      EWS-ERG fusion transcript. Subsequent two-color fluorescence in situ 
      hybridization (FISH) analysis with EWS and ERG clones showed the fused signals on 
      the der(21) chromosome, but no ERG signals on the chromosome 22 homologs. Thus, 
      our RT-PCR and FISH analyses indicated that the chromosome 22 fragment containing 
      the 5' portion of EWS had been inverted and inserted into chromosome 21 and had 
      fused to the 3' portion of ERG. This subtle chromosome aberration could not be 
      identified by routine cytogenetics. A chromosomal inversion/insertion has also 
      been described in acute leukemia with the MLL-AF10 fusion gene, and this may be a 
      common pathway for producing fusion of reverse-oriented genes in leukemias and 
      solid tumors.
FAU - Kaneko, Y
AU  - Kaneko Y
AD  - Department of Cancer Chemotherapy, Saitama Cancer Center Hospital, Japan.
FAU - Kobayashi, H
AU  - Kobayashi H
FAU - Handa, M
AU  - Handa M
FAU - Satake, N
AU  - Satake N
FAU - Maseki, N
AU  - Maseki N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Heterogeneous-Nuclear Ribonucleoproteins)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (RNA-Binding Protein EWS)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Ribonucleoproteins)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Bone Neoplasms/*genetics
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - Chromosome Inversion
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Chromosomes, Human, Pair 21/*genetics
MH  - Chromosomes, Human, Pair 22/*genetics
MH  - DNA, Neoplasm/analysis
MH  - Heterogeneous-Nuclear Ribonucleoproteins
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mutation
MH  - Polymerase Chain Reaction
MH  - RNA, Neoplasm/analysis
MH  - RNA-Binding Protein EWS
MH  - RNA-Binding Proteins/*genetics
MH  - RNA-Directed DNA Polymerase
MH  - Ribonucleoproteins/*genetics
MH  - Sarcoma, Ewing/*genetics
MH  - Sequence Analysis, DNA
MH  - Translocation, Genetic
EDAT- 1997/03/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - 10.1002/(SICI)1098-2264(199703)18:3<228::AID-GCC9>3.0.CO;2-3 [pii]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1997 Mar;18(3):228-31.