PMID- 9071740
OWN - NLM
STAT- MEDLINE
DCOM- 19970613
LR  - 20190822
IS  - 0039-128X (Print)
IS  - 0039-128X (Linking)
VI  - 62
IP  - 3
DP  - 1997 Mar
TI  - Presence of 11 beta-hydroxysteroid dehydrogenase in human semen: evidence of 
      correlation with semen characteristics.
PG  - 311-4
AB  - 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD), the enzyme that catalyzes the 
      conversion of biologically active glucocorticoids to their inactive metabolites, 
      was shown to be located exclusively in Leydig cells of the rat testis, and its 
      appearance was associated with the developmental rise in testosterone. Thus, 11 
      beta-HSD was suggested to play an important role in maintaining steroidogenesis 
      by inactivating excess cortisol that inhibits testosterone production. Whether 
      equivalent protection from glucocorticoids excess is necessary for 
      spermatogenesis is not known, and we have, accordingly, investigated the 11 
      beta-HSD activity in ejaculated human semen. Both 11 beta-dehydrogenase (11 
      beta-DH) and 11 beta-oxoreductase (11-OR) activities of 11 beta-HSD were 
      measurable in semen, although seminal plasma was devoid of 11 beta-HSD activity. 
      Azoospermic specimens were associated with low 11 beta-dehydrogenase activity, 
      indicating the presence of enzyme activity in cells other than spermatozoa. Pure 
      spermatozoa separated on percoll gradient could oxidize corticosterone in the 
      presence of NAD or NADP. Significantly higher 11 beta-DH activity is associated 
      with semen specimens with low sperm count (p < .05) and higher level of 
      morphologically abnormal spermatozoa (p < .05). The presence of 11 beta-HSD in 
      human semen and its association with sperm characteristics thus suggests 
      functional role for glucocorticoid exclusion in the sperm maturation process.
FAU - Nacharaju, V L
AU  - Nacharaju VL
AD  - Department of Obstetrics and Gynecology, SUNY Health Science Center 11203-2098, 
      USA.
FAU - Muneyyirci-Delale, O
AU  - Muneyyirci-Delale O
FAU - Khan, N
AU  - Khan N
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Steroids
JT  - Steroids
JID - 0404536
RN  - 0U46U6E8UK (NAD)
RN  - 53-59-8 (NADP)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
RN  - FO4V44A3G3 (11-dehydrocorticosterone)
RN  - MM6384NG73 (Carbenoxolone)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Carbenoxolone/pharmacology
MH  - Corticosterone/analogs & derivatives/metabolism
MH  - Humans
MH  - Hydroxysteroid Dehydrogenases/*metabolism
MH  - Male
MH  - NAD/drug effects/metabolism
MH  - NADP/drug effects/metabolism
MH  - Oligospermia/enzymology
MH  - Semen/*enzymology/*physiology
MH  - Sperm Motility
MH  - Spermatozoa/enzymology
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - S0039-128X(96)00225-5 [pii]
AID - 10.1016/s0039-128x(96)00225-5 [doi]
PST - ppublish
SO  - Steroids. 1997 Mar;62(3):311-4. doi: 10.1016/s0039-128x(96)00225-5.