PMID- 9072004
OWN - NLM
STAT- MEDLINE
DCOM- 19970402
LR  - 20171116
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 181
IP  - 1
DP  - 1997 Jan
TI  - Chromosomal rearrangement t(11;22) in extraskeletal Ewing's sarcoma and primitive 
      neuroectodermal tumour analysed by fluorescence in situ hybridization using 
      paraffin-embedded tissue.
PG  - 62-6
AB  - The clonal chromosomal rearrangement t(11;22) has been reported by karyotypic 
      analysis to be specific for Ewing's sarcoma of bone and soft tissue origin as 
      well as primitive neuroectodermal tumour. In this report, immunohistological 
      analysis of MIC 2 expression and fluorescence in situ hybridization (FISH) were 
      performed using paraffin-embedded tissues. We examined t(11;22) in the nuclei 
      isolated from two Ewing's sarcomas, four primitive neuroectodermal tumours, and 
      three neuroblastomas, which served as negative controls by FISH with an 
      alpha-satellite DNA probe for chromosome 11, a chromosome 22 marker probe, and 
      whole chromosome painting probes for both chromosomes 11 and 22. Both cases of 
      Ewing's sarcoma and the four primitive neuroectodermal tumour specimens were 
      immunoreactive for MIC 2. Both Ewing's sarcomas and three of the four primitive 
      neuroectodermal tumours contained the tumour-specific t(11;22), but the three 
      neuroblastomas did not show this translocation. Based on the cytogenetic results 
      and on the immunohistological investigation of MIC 2 expression, Ewing's sarcoma 
      is suggested to be related closely to primitive neuroectodermal tumour. FISH is a 
      useful aid in determining the tumour type of Ewing's sarcoma and putative related 
      tumours.
FAU - Nagao, K
AU  - Nagao K
AD  - Department of Pathology, Faculty of Medicine, Tottori University, Japan.
FAU - Ito, H
AU  - Ito H
FAU - Yoshida, H
AU  - Yoshida H
FAU - Minamizaki, T
AU  - Minamizaki T
FAU - Furuse, K
AU  - Furuse K
FAU - Yoshikawa, T
AU  - Yoshikawa T
FAU - Ushigome, S
AU  - Ushigome S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (12E7 Antigen)
RN  - 0 (Antigens, CD)
RN  - 0 (CD99 protein, human)
RN  - 0 (Cell Adhesion Molecules)
SB  - IM
MH  - 12E7 Antigen
MH  - Adolescent
MH  - Adult
MH  - Antigens, CD/metabolism
MH  - Cell Adhesion Molecules/metabolism
MH  - *Chromosomes, Human, Pair 11
MH  - *Chromosomes, Human, Pair 22
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Male
MH  - Middle Aged
MH  - Neuroblastoma/genetics
MH  - Neuroectodermal Tumors, Primitive/genetics
MH  - Paraffin Embedding
MH  - Sarcoma, Ewing/*genetics/metabolism
MH  - Soft Tissue Neoplasms/*genetics/metabolism
MH  - *Translocation, Genetic
EDAT- 1997/01/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1002/(SICI)1096-9896(199701)181:1<62::AID-PATH687>3.0.CO;2-P [pii]
AID - 10.1002/(SICI)1096-9896(199701)181:1<62::AID-PATH687>3.0.CO;2-P [doi]
PST - ppublish
SO  - J Pathol. 1997 Jan;181(1):62-6. doi: 
      10.1002/(SICI)1096-9896(199701)181:1<62::AID-PATH687>3.0.CO;2-P.