PMID- 9072984
OWN - NLM
STAT- MEDLINE
DCOM- 19970603
LR  - 20130523
IS  - 0903-1936 (Print)
IS  - 0903-1936 (Linking)
VI  - 10
IP  - 3
DP  - 1997 Mar
TI  - MCP-1 secretion in lung from nonsmoking patients with coal worker's 
      pneumoconiosis.
PG  - 557-62
AB  - Exposure to coal dust leads to the development of coal worker's pneumoconiosis 
      (CWP), a disease associated with an accumulation of macrophages in the lower 
      respiratory tract. Mechanisms controlling monocyte recruitment are still poorly 
      understood. Since monocyte chemoattractant protein-1 (MCP-1) is recognized as a 
      potent chemotactic factor for blood monocytes, we analysed the presence of MCP-1 
      in the pulmonary compartment of patients with CWP. Bronchoalveolar lavage fluid 
      (BALF) from 16 nonsmoking control subjects and 27 nonsmoking CWP patients (16 
      with simple pneumoconiosis (SP) and 11 with progressive massive fibrosis (PMF)) 
      was analysed. Alveolar macrophages (AMs) were purified by adherence and BALF was 
      concentrated tenfold by lyophilization. MCP-1 was measured in BALF and in 3 h AM 
      supernatants using a sandwich enzyme-linked immunosorbent assay (ELISA). The 
      localization of MCP-1 in lung tissue was determined by immunohistochemistry on 
      tissue sections from three patients with CWP and two control subjects. MCP-1 
      levels were significantly higher in concentrated BALF from patients with SP or 
      PMF (median 370 and 555 pg x mL-1, respectively) than in those from control 
      subjects (median 11 pg x mL-1) (p<0.001). Released MCP-1 in AM supernatants was 
      enhanced in patients with CWP (median 83 pg x mL-1) but compared to controls 
      (median 41 pg x mL-1) this level did not reach significance. Although 
      significantly increased, AM counts in BALF from patients with CWP did not 
      correlate with MCP-1 levels. MCP-1 levels in BALF correlated with MCP-1 levels in 
      AM supernatants (p=0.47; p<0.02). In control lung specimens, MCP-1 was expressed 
      by a few AMs, type II pneumocytes and perivascular smooth muscle cells. CWP 
      sections were characterized by an increased number of AMs and mainly by the 
      presence of fibroblasts (in the myogenic area of fibrotic lesions) and 
      hyperplastic type II pneumocytes, which were strongly immunostained for MCP-1. 
      Our data demonstrate that: 1) patients with coal worker's pneumoconiosis have a 
      marked pulmonary overproduction of monocyte chemoattractant protein-1; and 2) in 
      addition to alveolar macrophages, fibroblasts (probably myofibroblasts) and 
      hyperplastic type II pneumocytes may also be responsible for this increased level 
      of monocyte chemoattractant protein-1 in coal worker's pneumoconiosis.
FAU - Boitelle, A
AU  - Boitelle A
AD  - INSERM U416, Institut Pasteur, Lille, France.
FAU - Gosset, P
AU  - Gosset P
FAU - Copin, M C
AU  - Copin MC
FAU - Vanhee, D
AU  - Vanhee D
FAU - Marquette, C H
AU  - Marquette CH
FAU - Wallaert, B
AU  - Wallaert B
FAU - Gosselin, B
AU  - Gosselin B
FAU - Tonnel, A B
AU  - Tonnel AB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur Respir J
JT  - The European respiratory journal
JID - 8803460
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Aged
MH  - Bronchoalveolar Lavage Fluid/chemistry
MH  - Case-Control Studies
MH  - Chemokine CCL2/*metabolism
MH  - *Coal Mining
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immunohistochemistry
MH  - Lung/*metabolism/pathology
MH  - Macrophages, Alveolar/metabolism
MH  - Middle Aged
MH  - Pneumoconiosis/diagnosis/*metabolism/pathology
MH  - Smoking
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
PST - ppublish
SO  - Eur Respir J. 1997 Mar;10(3):557-62.