PMID- 9073510
OWN - NLM
STAT- MEDLINE
DCOM- 19970505
LR  - 20220311
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 40
IP  - 3
DP  - 1997 Mar 15
TI  - Structural organization of the human prostaglandin EP3 receptor subtype gene 
      (PTGER3).
PG  - 425-34
AB  - Prostaglandin EP3 receptor subtype is a seven-membrane-spanning protein with 
      multiple C-terminal tails generated by alternative mRNA splicing. We report here 
      the structural organization of the human EP3 gene (PTGER3). The human EP3 gene 
      spanned more than 80 kb and was composed of 10 exons separated by nine introns. 
      Exon 1 and the 5' 180-bp portion of exon 2 (exon 2a) encoded the seven 
      transmembrane domains and 10 amino acid residues of the cytoplasmic tail, which 
      are common to all EP3 isoforms. The 3' 3461-bp portion of exon 2 (exon 2b) or 
      combinations of exons 3-10 encoded the EP3 isoform-specific C termini and formed 
      their 3'-untranslated regions by multiple fashions of alternative mRNA splicing. 
      Exons 2b, 4, 6, and 10 contained polyadenylation sites. The EP3 gene formed nine 
      distinct mRNAs encoding eight EP3 isoforms, two of which were novel ones 
      tentatively designated EP3-V and EP3-VI. The transcription initiation sites of 
      the human EP3 gene were mapped 227 to approximately 231 bp upstream of the ATG 
      start codon. The 360-bp 5'-flanking region contained a TATA box-like sequence, a 
      GC box, and several cis-acting regulatory elements. The present study provides 
      insight into the molecular mechanisms underlying the prostanoid receptor family.
FAU - Kotani, M
AU  - Kotani M
AD  - Department of Medicine, Kyoto University Graduate School of Medicine, Kyoto, 606, 
      Japan.
FAU - Tanaka, I
AU  - Tanaka I
FAU - Ogawa, Y
AU  - Ogawa Y
FAU - Usui, T
AU  - Usui T
FAU - Tamura, N
AU  - Tamura N
FAU - Mori, K
AU  - Mori K
FAU - Narumiya, S
AU  - Narumiya S
FAU - Yoshimi, T
AU  - Yoshimi T
FAU - Nakao, K
AU  - Nakao K
LA  - eng
SI  - GENBANK/D86087
SI  - GENBANK/D86088
SI  - GENBANK/D86089
SI  - GENBANK/D86090
SI  - GENBANK/D86091
SI  - GENBANK/D86092
SI  - GENBANK/D86093
SI  - GENBANK/D86094
SI  - GENBANK/D86095
SI  - GENBANK/D86096
SI  - GENBANK/D86097
SI  - GENBANK/D86098
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (PTGER3 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Prostaglandin E)
RN  - 0 (Receptors, Prostaglandin E, EP3 Subtype)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Humans
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics
MH  - Receptors, Prostaglandin E/*genetics
MH  - Receptors, Prostaglandin E, EP3 Subtype
MH  - Sequence Alignment
MH  - Sequence Analysis
EDAT- 1997/03/15 00:00
MHDA- 1997/03/15 00:01
CRDT- 1997/03/15 00:00
PHST- 1997/03/15 00:00 [pubmed]
PHST- 1997/03/15 00:01 [medline]
PHST- 1997/03/15 00:00 [entrez]
AID - S0888-7543(96)94585-1 [pii]
AID - 10.1006/geno.1996.4585 [doi]
PST - ppublish
SO  - Genomics. 1997 Mar 15;40(3):425-34. doi: 10.1006/geno.1996.4585.