PMID- 9075485
OWN - NLM
STAT- MEDLINE
DCOM- 19970612
LR  - 20191101
IS  - 0889-2229 (Print)
IS  - 0889-2229 (Linking)
VI  - 13
IP  - 5
DP  - 1997 Mar 20
TI  - Simultaneous detection of simian retrovirus type D serotypes 1, 2, and 3 by 
      polymerase chain reaction.
PG  - 433-7
AB  - Asymptomatic infection of macaques with macaques with simian retroviruses type D 
      (SRV/D), the etiologic agents of one form of retrovirus-induced simian 
      immunodeficiency disease, can confound experiments with the simian 
      immunodeficiency virus (SIV), which also induces immunodeficiency disease in 
      macaques. The SIV/macaque model is the preferred nonhuman primate model for 
      AIDS-related research. Serological screening for SRV/D alone is insufficient 
      because not all infected animals seroconvert, and virus isolation by 
      cocultivation may require 4 to 6 weeks. We have established a DNA polymerase 
      chain reaction (PCR) assay. One set of nested primers allows detection of SRV/D 
      serotypes 1, 2, and 3 and distinguishes SRV-2 from the other two serotypes. The 
      PCR assay is sensitive; a single proviral copy of SRV/D could be detected in 
      150,000 to 210,000 macaque peripheral blood mononuclear cells (PBMCs). When 
      applied to a panel of virus isolation-positive macaque samples, the PCR assay was 
      positive in 100% of the tests. No false-positive results were seen when known 
      specific-pathogen-free (SPF) macaques were examined. We propose that macaques be 
      screened with a combination of SRV/D serology and this DNA PCR assay prior to 
      enrollment in experiments with SIV.
FAU - Liska, V
AU  - Liska V
AD  - Laboratory of Viral Pathogenesis, Dana-Farber Cancer Institute, Boston, 
      Massachusetts 02115, USA.
FAU - Lerche, N W
AU  - Lerche NW
FAU - Ruprecht, R M
AU  - Ruprecht RM
LA  - eng
GR  - IR01-AI32330-05/AI/NIAID NIH HHS/United States
GR  - R01-AI35533-2/AI/NIAID NIH HHS/United States
GR  - RR00169/RR/NCRR NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
RN  - 0 (Gene Products, env)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - DNA, Viral/*blood
MH  - Female
MH  - Gene Products, env/genetics
MH  - Leukocytes, Mononuclear/virology
MH  - Macaca fascicularis/*virology
MH  - Male
MH  - Polymerase Chain Reaction/*methods
MH  - Proviruses/isolation & purification
MH  - Retroviruses, Simian/genetics/immunology/*isolation & purification
MH  - Sensitivity and Specificity
MH  - Serotyping
MH  - Simian Acquired Immunodeficiency Syndrome/*diagnosis
EDAT- 1997/03/20 00:00
MHDA- 1997/03/20 00:01
CRDT- 1997/03/20 00:00
PHST- 1997/03/20 00:00 [pubmed]
PHST- 1997/03/20 00:01 [medline]
PHST- 1997/03/20 00:00 [entrez]
AID - 10.1089/aid.1997.13.433 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 1997 Mar 20;13(5):433-7. doi: 10.1089/aid.1997.13.433.