PMID- 9075826
OWN - NLM
STAT- MEDLINE
DCOM- 19970415
LR  - 20180215
IS  - 1018-1172 (Print)
IS  - 1018-1172 (Linking)
VI  - 34
IP  - 1
DP  - 1997 Jan-Feb
TI  - Monocyte chemotactic protein 1 (MCP-1) is a mitogen for cultured rat vascular 
      smooth muscle cells.
PG  - 58-65
AB  - The involvement of inflammatory mechanisms in the progression of atherosclerosis 
      has recently been suggested. Monocyte chemotactic protein 1 (MCP-1) is a soluble 
      protein which is implicated in acute and chronic inflammatory processes, 
      including atherosclerosis. We evaluated the effect of human recombinant MCP-1 on 
      the in vitro proliferation of rat vascular smooth muscle cells (VSMCs). 
      Incubation of VSMCs with MCP-1 (50-200 ng/ml) in the presence of 0.5% FCS 
      significantly increased cell proliferation, [3H]-thymidine incorporation and the 
      proliferative S fraction, measured by flow cytometry, compared to control cells. 
      The proliferative effect of MCP-1 was specific, as shown by inhibition with a 
      rabbit polyclonal serum to MCP-1. Moreover, the mitogenic effect of MCP-1 was 
      significantly inhibited by downregulation of protein kinase C (PKC) activity and 
      by incubation with H-7, a protein kinase inhibitor, suggesting the involvement of 
      the PKC system. Verapamil, a Ca2+ channel blocker, also reduced the stimulatory 
      effect of MCP-1 on cell proliferation. This study demonstrates that MCP-1 does 
      not merely have a chemotactic activity, but also a mitogenic effect on cultured 
      rat VSMCs.
FAU - Porreca, E
AU  - Porreca E
AD  - Istituto di Medicina Interna, Facolta di Medicina e Chirurgia, Universita di 
      Chieti, Italy.
FAU - Di Febbo, C
AU  - Di Febbo C
FAU - Reale, M
AU  - Reale M
FAU - Castellani, M L
AU  - Castellani ML
FAU - Baccante, G
AU  - Baccante G
FAU - Barbacane, R
AU  - Barbacane R
FAU - Conti, P
AU  - Conti P
FAU - Cuccurullo, F
AU  - Cuccurullo F
FAU - Poggi, A
AU  - Poggi A
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - J Vasc Res
JT  - Journal of vascular research
JID - 9206092
RN  - 0 (Chemokine CCL2)
RN  - 0 (Mitogens)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/*pharmacology
MH  - Humans
MH  - Mitogens/*pharmacology
MH  - Muscle, Smooth, Vascular/cytology/*drug effects
MH  - Rabbits
MH  - Rats
MH  - Recombinant Proteins/pharmacology
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1159/000159202 [doi]
PST - ppublish
SO  - J Vasc Res. 1997 Jan-Feb;34(1):58-65. doi: 10.1159/000159202.