PMID- 9076997
OWN - NLM
STAT- MEDLINE
DCOM- 19970627
LR  - 20231213
IS  - 0960-7412 (Print)
IS  - 0960-7412 (Linking)
VI  - 11
IP  - 2
DP  - 1997 Feb
TI  - Identification and functional significance of a new class of non-consensus-type 
      plastid promoters.
PG  - 327-37
AB  - The promoter regions of most plastid transcription units have been reported to 
      consist of prokaryotic -10 and -35-like consensus sequences. However, a few 
      promoters with no homology to the consensus elements have also been 
      characterized. A novel class of non-consensus plastid promoters--designated as 
      non consensus type II (NC-II) promoters--that effect low-level constitutive 
      expression of respective genes in photosynthetic as well as non-photosynthetic 
      plastids is described in this paper. The abundance of NC-II promoter-derived 
      transcripts remains unaltered even when light-grown seedlings are shifted to the 
      dark. In contrast, transcripts from -10 and -35-like elements containing 
      consensus type (CT) promoters accumulate to high levels in chloroplasts as 
      compared with non-photosynthetic plastids of roots and cultured cells. Moreover, 
      accumulation of these transcripts is greatly affected by light. The inhibition of 
      plastid protein synthesis has no apparent effect on the abundance of the NC-II 
      transcripts whereas levels of CT transcripts are greatly reduced. In vivo 
      tagetitoxin (a plastid transcription inhibitor) treatment also reduces the levels 
      of CT transcripts with no apparent inhibitory effect on the accumulation of NC-II 
      transcripts. The accumulation of transcripts from both classes of promoters, 
      however, is reduced when cytoplasmic protein synthesis is inhibited by in vivo 
      treatment with cycloheximide. The results are suggestive of the possible 
      existence of at least two distinctive systems for the synthesis and/or 
      maintenance of plastid transcripts which differentiate between two classes of 
      transcripts in a promoter-type specific manner.
FAU - Kapoor, S
AU  - Kapoor S
AD  - Center for Gene Research, Nagoya University, Japan.
FAU - Suzuki, J Y
AU  - Suzuki JY
FAU - Sugiura, M
AU  - Sugiura M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (Dicarboxylic Acids)
RN  - 0 (Organophosphorus Compounds)
RN  - 0 (Plant Proteins)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Plant)
RN  - 87913-21-1 (tagetitoxin)
RN  - 98600C0908 (Cycloheximide)
SB  - IM
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Consensus Sequence/genetics
MH  - Cycloheximide/pharmacology
MH  - Darkness
MH  - Dicarboxylic Acids/pharmacology
MH  - Gene Expression Regulation, Plant/radiation effects
MH  - Light
MH  - Molecular Sequence Data
MH  - Organophosphorus Compounds/pharmacology
MH  - Plant Proteins/biosynthesis
MH  - Plants, Toxic
MH  - Plastids/*genetics
MH  - Promoter Regions, Genetic/*genetics
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - RNA, Messenger/biosynthesis
MH  - RNA, Plant/biosynthesis
MH  - Nicotiana/genetics
MH  - Transcription, Genetic/drug effects
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - 10.1046/j.1365-313x.1997.11020327.x [doi]
PST - ppublish
SO  - Plant J. 1997 Feb;11(2):327-37. doi: 10.1046/j.1365-313x.1997.11020327.x.