PMID- 9077472
OWN - NLM
STAT- MEDLINE
DCOM- 19970409
LR  - 20190723
IS  - 0022-202X (Print)
IS  - 0022-202X (Linking)
VI  - 108
IP  - 4
DP  - 1997 Apr
TI  - The chemokine repertoire of human dermal microvascular endothelial cells and its 
      regulation by inflammatory cytokines.
PG  - 445-51
AB  - Activation of endothelium is a critical event during the initiation of 
      inflammatory processes and is associated with the induction of cell adhesion 
      molecules and cytokines. The latter include chemotactically active cytokines 
      (chemokines) that promote leukocyte diapedesis from the circulation to sites of 
      evolving inflammation. In this study we evaluated the chemokine repertoire of 
      human endothelial cells derived from the skin (HDMECs) and regulation of these 
      chemokines by cytokines. HDMECs and an immortalized human dermal microvascular 
      endothelial cell line, HMEC-1, were investigated for the expression of C-X-C and 
      C-C chemokines at mRNA and protein levels. Upon stimulation with 
      interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha), both 
      HDMECs and HMEC-1 expressed high levels of IL-8, GRO, and monocyte 
      chemoattractant protein-1 (MCP-1). RANTES was only weakly induced; however, 
      concomitant treatment with TNF-alpha and interferon-gamma (IFN-gamma) led to 
      upregulation of RANTES, indicating a synergy between these two cytokines. The 
      C-X-C chemokine IFN-inducible protein-10 was upregulated by IFN-gamma but not by 
      other cytokines studied. Macrophage inflammatory protein-1alpha and beta, 1-309, 
      and ENA-78 could not be induced. The chemokine repertoires of HDMECs and HMEC-1 
      were compared to those of human umbilical vein endothelium and found to be rather 
      similar with the important exception that IFN-gamma and IL-4 up-regulated MCP-1 
      only in macrovascular endothelium. Our data indicate that HDMECs contribute to 
      the dermal cytokine network by selective production of MCP-1, IL-8, GRO, RANTES, 
      and IP-10, which may critically influence the site-specific recruitment of 
      leukocyte subsets.
FAU - Goebeler, M
AU  - Goebeler M
AD  - Department of Dermatology, University of Wurzburg, Germany.
FAU - Yoshimura, T
AU  - Yoshimura T
FAU - Toksoy, A
AU  - Toksoy A
FAU - Ritter, U
AU  - Ritter U
FAU - Brocker, E B
AU  - Brocker EB
FAU - Gillitzer, R
AU  - Gillitzer R
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (Chaperonin 10)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Cells, Cultured
MH  - Chaperonin 10/biosynthesis/genetics
MH  - Chemokine CCL2/biosynthesis/genetics
MH  - Chemokine CCL5/biosynthesis
MH  - Chemokines/*metabolism
MH  - Cytokines/*pharmacology
MH  - Endothelium, Vascular/chemistry/*cytology/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - In Situ Hybridization
MH  - Infant, Newborn
MH  - Interleukin-1/pharmacology
MH  - Interleukin-4/pharmacology
MH  - Interleukin-8/biosynthesis/genetics
MH  - Male
MH  - Microcirculation/cytology
MH  - RNA, Messenger/metabolism
MH  - Skin/chemistry/*cytology
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Umbilical Veins/cytology
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - S0022-202X(15)42852-0 [pii]
AID - 10.1111/1523-1747.ep12289711 [doi]
PST - ppublish
SO  - J Invest Dermatol. 1997 Apr;108(4):445-51. doi: 10.1111/1523-1747.ep12289711.