PMID- 9078291
OWN - NLM
STAT- MEDLINE
DCOM- 19970410
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 94
IP  - 1
DP  - 1997 Mar
TI  - Interphase cytogenetics in chronic lymphocytic leukemia.
PG  - 52-8
AB  - The incidence of trisomy 12 and 13q12-q14 abnormalities in patients with chronic 
      lymphocytic leukemia (CLL) was determined by conventional cytogenetics and 
      interphase fluorescence in situ hybridization (FISH). In the analysis of 580 
      consecutive patients, trisomy 12 was detected by conventional cytogenetics in 39 
      cases (9%) and 117 cases (20%) by FISH. Trisomy 12 was shown to be associated 
      with advanced clinical stage, atypical morphology, and higher proliferative 
      activity. Combined immunophenotyping and FISH showed that trisomy 12 was present 
      only in a proportion of the clonal B-cells. These data suggest that trisomy 12 is 
      a secondary event associated with features of disease progression. Sequential 
      FISH showed clonal progression of the trisomic clone over time. Three hundred 
      patients also were investigated for 13q deletions using FISH analysis of the RB1 
      locus (13q14). Monoallelic RB1 deletion was seen in 104 (34%) of cases. One case 
      had a homozygous deletion in 90% of the cells. Dual-color FISH detected the 
      presence of trisomy 12 and RB1 in 17 (5%) cases. DNA probes for 13q12.3 (BRCA2) 
      and 13q14 (RB1 and DBM locus) were used in 35 cases. Twenty-eight (80%) cases 
      showed deletion of a 1Mb 13q12.3 encompassing the BRCA2 locus, whereas 22/35 
      (63%) were deleted at 13q14. Our data suggest that abnormalities of 13q are more 
      frequent than trisomy 12 in CLL and provide evidence for the presence of a new 
      candidate gene at 13q12.3 that may be involved in the pathogenesis of CLL.
FAU - Garcia-Marco, J A
AU  - Garcia-Marco JA
AD  - Academic Department of Hematology and Cytogenetics, Royal Marsden Hospital, 
      London, UK.
FAU - Price, C M
AU  - Price CM
FAU - Catovsky, D
AU  - Catovsky D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - *Chromosome Deletion
MH  - Chromosomes, Human, Pair 12/*genetics
MH  - Chromosomes, Human, Pair 13/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics
MH  - Trisomy/*genetics
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - S0165460896003044 [pii]
AID - 10.1016/s0165-4608(96)00304-4 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1997 Mar;94(1):52-8. doi: 10.1016/s0165-4608(96)00304-4.