PMID- 9079689
OWN - NLM
STAT- MEDLINE
DCOM- 19970502
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 272
IP  - 13
DP  - 1997 Mar 28
TI  - Characterization of a subset of the basic-helix-loop-helix-PAS superfamily that 
      interacts with components of the dioxin signaling pathway.
PG  - 8581-93
AB  - In an effort to better understand the mechanism of toxicity of 2,3,7, 
      8-tetrachlorodibenzo-p-dioxin, we employed an iterative search of human expressed 
      sequence tags to identify novel basic-helix-loop-helix-PAS (bHLH-PAS) proteins 
      that interact with either the Ah receptor (AHR) or the Ah receptor nuclear 
      translocator (ARNT). We characterized five new "members of the PAS superfamily," 
      or MOPs 1-5, that are similar in size and structural organization to the AHR and 
      ARNT. MOPs 1-4 have N-terminal bHLH and PAS domains and C-terminal variable 
      regions. MOP5 contained the characteristic PAS domain and a variable C terminus; 
      it is possible that the cDNA contains a bHLH domain, but the entire open reading 
      frame has yet to be completed. Coimmunoprecipitation studies, yeast two-hybrid 
      analysis, and transient transfection experiments demonstrated that MOP1 and MOP2 
      dimerize with ARNT and that these complexes are transcriptionally active at 
      defined DNA enhancer sequences in vivo. MOP3 was found to associate with the AHR 
      in vitro but not in vivo. This observation, coupled with the fact that MOP3 
      formed tighter associations with the 90-kDa heat shock protein than the human 
      AHR, suggests that MOP3 may be a conditionally active bHLH-PAS protein that 
      requires activation by an unknown ligand. The expression profiles of the AHR, 
      MOP1, and MOP2 mRNAs, coupled with the observation that they all share ARNT as a 
      common dimeric partner, suggests that the cellular pathways mediated by MOP1 and 
      MOP2 may influence or respond to the dioxin signaling pathway.
FAU - Hogenesch, J B
AU  - Hogenesch JB
AD  - Department of Molecular Pharmacology and Biological Chemistry, Northwestern 
      University Medical School, Chicago, Illinois 60611, USA.
FAU - Chan, W K
AU  - Chan WK
FAU - Jackiw, V H
AU  - Jackiw VH
FAU - Brown, R C
AU  - Brown RC
FAU - Gu, Y Z
AU  - Gu YZ
FAU - Pray-Grant, M
AU  - Pray-Grant M
FAU - Perdew, G H
AU  - Perdew GH
FAU - Bradfield, C A
AU  - Bradfield CA
LA  - eng
SI  - GENBANK/AF044288
SI  - GENBANK/U29165
SI  - GENBANK/U51625
SI  - GENBANK/U51626
SI  - GENBANK/U51627
SI  - GENBANK/U51628
GR  - ES05660/ES/NIEHS NIH HHS/United States
GR  - ES05703/ES/NIEHS NIH HHS/United States
GR  - P30-CA07175/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (ARNT protein, human)
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Polychlorinated Dibenzodioxins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 0 (Transcription Factors)
RN  - 0 (sim protein, Drosophila)
RN  - 138391-32-9 (Aryl Hydrocarbon Receptor Nuclear Translocator)
SB  - IM
MH  - Amino Acid Sequence
MH  - Aryl Hydrocarbon Receptor Nuclear Translocator
MH  - Basic Helix-Loop-Helix Transcription Factors
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry
MH  - DNA-Binding Proteins/*metabolism
MH  - Drosophila Proteins
MH  - *Helix-Loop-Helix Motifs
MH  - Humans
MH  - Hypoxia-Inducible Factor 1
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/*metabolism
MH  - Open Reading Frames
MH  - Polychlorinated Dibenzodioxins/*pharmacology
MH  - RNA, Messenger/metabolism
MH  - Receptors, Aryl Hydrocarbon/*metabolism
MH  - Sequence Alignment
MH  - Software
MH  - Transcription Factors/*metabolism
EDAT- 1997/03/28 00:00
MHDA- 1997/03/28 00:01
CRDT- 1997/03/28 00:00
PHST- 1997/03/28 00:00 [pubmed]
PHST- 1997/03/28 00:01 [medline]
PHST- 1997/03/28 00:00 [entrez]
AID - S0021-9258(18)35514-5 [pii]
AID - 10.1074/jbc.272.13.8581 [doi]
PST - ppublish
SO  - J Biol Chem. 1997 Mar 28;272(13):8581-93. doi: 10.1074/jbc.272.13.8581.