PMID- 9084855
OWN - NLM
STAT- MEDLINE
DCOM- 19970613
LR  - 20190920
IS  - 1068-9265 (Print)
IS  - 1068-9265 (Linking)
VI  - 4
IP  - 2
DP  - 1997 Mar
TI  - Apoptosis induced in human colorectal carcinoma by anti-Fas antibody.
PG  - 169-75
AB  - BACKGROUND: Apoptosis or programmed cell death has been shown to play an 
      important role in the progression from polyps to carcinomas. Fas/APO-1 is a cell 
      surface protein that can induce apoptosis in a variety of cell types upon 
      specific binding. In this study seven human colorectal carcinoma (HCRC) cell 
      lines of varying differentiation were analyzed for cell surface Fas expression. 
      Fas-mediated apoptosis, and correlation of apoptosis with bcl-2 expression. 
      METHODS AND RESULTS: Using flow cytometry, all seven lines expressed varying 
      amounts of cell surface Fas antigen. Exposure to anti-Fas antibody induced cell 
      death in all the cell lines, albeit to varying degrees. The rate of apoptosis was 
      quantitated using flow cytometry with propidium iodide staining of nuclear DNA. 
      The poorly differentiated cell lines had a significantly decreased (p < 0.05) 
      anti-Fas sensitivity as compared with the well-differentiated lines. Measurement 
      of bcl-2 expression by flow cytometry showed an inverse correlation with anti-Fas 
      sensitivity. CONCLUSIONS: This study confirms that HCRC cell lines express Fas 
      antigen and, more importantly, provides the first evidence that exposure to 
      anti-Fas antibody can induce apoptosis. Fas-mediated apoptosis in HCRC cell lines 
      may be regulated by bcl-2 and may correlate with the degree of differentiation.
FAU - Meterissian, S H
AU  - Meterissian SH
AD  - Department of Surgery, Royal Victoria Hospital, McGill University, Montreal, 
      Quebec, Canada.
FAU - Kontogiannea, M
AU  - Kontogiannea M
FAU - Po, J
AU  - Po J
FAU - Jensen, G
AU  - Jensen G
FAU - Ferdinand, B
AU  - Ferdinand B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Ann Surg Oncol
JT  - Annals of surgical oncology
JID - 9420840
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (fas Receptor)
SB  - IM
MH  - Antibodies, Monoclonal/*immunology
MH  - *Apoptosis
MH  - Colorectal Neoplasms/*immunology
MH  - DNA, Neoplasm/analysis
MH  - Flow Cytometry
MH  - Humans
MH  - Proto-Oncogene Proteins c-bcl-2/analysis
MH  - Tumor Cells, Cultured
MH  - fas Receptor/analysis/*immunology
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - 10.1007/BF02303801 [doi]
PST - ppublish
SO  - Ann Surg Oncol. 1997 Mar;4(2):169-75. doi: 10.1007/BF02303801.