PMID- 9085736
OWN - NLM
STAT- MEDLINE
DCOM- 19970610
LR  - 20071115
IS  - 0268-3369 (Print)
IS  - 0268-3369 (Linking)
VI  - 19
IP  - 6
DP  - 1997 Mar
TI  - Usefulness of detection of minimal residual disease by 'hypermetaphase' 
      fluorescent in situ hybridization after allogeneic BMT for chronic myelogenous 
      leukemia.
PG  - 565-70
AB  - Hypermetaphase FISH (HMF), a molecular cytogenetic procedure combining the long 
      term mitotic arrest of bone marrow cultures with detection of a specific 
      chromosomal rearrangement by fluorescence in situ hybridization (FISH), has 
      recently been shown to be effective in determining the frequency of Philadelphia 
      chromosome positive (Ph+) cells in the bone marrow of chronic myelogenous 
      leukemia (CML) patients undergoing treatment. By combining the probe for the Ph 
      chromosome with one for the detection of the X chromosome, we used HMF to monitor 
      the presence of malignant cells within the emerging host cell population in the 
      marrow of a CML patient that had undergone sex-mismatched allogeneic bone marrow 
      transplantation. In successive studies, we detected 0.5% and 1.75% Ph+ cells, 
      respectively, confirmed by Western blot analysis for p210 protein. These readings 
      occurred concordantly with a repopulation of host-derived diploid female cells. 
      Standard G-band cytogenetic analyses did not detect any Ph+ cells at these time 
      points. Intervention with donor lymphocyte infusion reinduced complete remission. 
      This experience indicates that HMF is useful to identify low levels of 
      repopulation by Ph+ cells in the marrow post-BMT at a stage when intervention is 
      most efficacious.
FAU - Seong, D
AU  - Seong D
AD  - Section of Blood and Marrow Transplantation, University of Texas, MD Anderson 
      Cancer Center, Houston, USA.
FAU - Giralt, S
AU  - Giralt S
FAU - Fischer, H
AU  - Fischer H
FAU - Hayes, K
AU  - Hayes K
FAU - Glassman, A
AU  - Glassman A
FAU - Arlinghaus, R
AU  - Arlinghaus R
FAU - Xu, J
AU  - Xu J
FAU - Kantarjian, H
AU  - Kantarjian H
FAU - Siciliano, M
AU  - Siciliano M
FAU - Champlin, R
AU  - Champlin R
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - England
TA  - Bone Marrow Transplant
JT  - Bone marrow transplantation
JID - 8702459
RN  - 0 (ABO Blood-Group System)
SB  - IM
MH  - ABO Blood-Group System
MH  - Adult
MH  - Bone Marrow/*ultrastructure
MH  - *Bone Marrow Transplantation
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL 
      Positive/blood/genetics/pathology/*therapy
MH  - Transplantation, Homologous
EDAT- 1997/03/02 00:00
MHDA- 1997/03/02 00:01
CRDT- 1997/03/02 00:00
PHST- 1997/03/02 00:00 [pubmed]
PHST- 1997/03/02 00:01 [medline]
PHST- 1997/03/02 00:00 [entrez]
AID - 10.1038/sj.bmt.1700700 [doi]
PST - ppublish
SO  - Bone Marrow Transplant. 1997 Mar;19(6):565-70. doi: 10.1038/sj.bmt.1700700.