PMID- 9087919
OWN - NLM
STAT- MEDLINE
DCOM- 19970626
LR  - 20190522
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 9
IP  - 1
DP  - 1997 Jan
TI  - Immunohistochemical detection of hog cholera viral glycoprotein 55 in 
      paraffin-embedded tissues.
PG  - 10-6
AB  - Formalin-fixed, paraffin embedded tissues obtained from 40 pigs inoculated with a 
      field isolate of hog cholera virus were examined for the presence of Gp55, a 
      major structural protein of the virus envelope, using a monoclonal antibody-based 
      immunohistochemical test with the avidin-biotin-peroxidase complex method. 
      Immunoreactivity was detected in hog cholera virus-infected tissues but not in 
      control pigs tissues, African swine fever virus-infected tissues, or bovine viral 
      diarrhea virus-infected porcine or bovine tissues. The first positive reactions 
      were seen in lymphatic tissues, digestive tract and skin on postinoculation day 
      (pid) 4, respiratory and urinary tissues on pid 5, nervous tissues on pid 6, and 
      endocrine tissues on pid 7. These staining reactions persisted until the last 
      observation on pid 18. Hog cholera virus antigen was not detected in heart tissue 
      at any time. The highest levels of antigen detection were found in tonsils, 
      spleen, and pancreas, although the esophageal mucosa and skin epithelial cells 
      were also intensely and widely stained. The cellular staining pattern of Gp55 had 
      a ubiquitous distribution. It was found in epithelial cells, macrophages and 
      circulating monocytes, endothelial cells, lymphoid cells, and glial cells. The 
      results showed a high specificity and high sensitivity for detecting hog cholera 
      Gp55 in formalin-fixed, paraffin embedded tissue samples. This method allows 
      precise association of Gp55 with specific cells, tissues, and histologic lesions, 
      making the technique suitable for use in routine diagnosis of hog cholera.
FAU - de las Mulas, J M
AU  - de las Mulas JM
AD  - Department of Comparative Pathology, Veterinary Faculty, University of Cordoba, 
      Spain.
FAU - Ruiz-Villamor, E
AU  - Ruiz-Villamor E
FAU - Donoso, S
AU  - Donoso S
FAU - Quezada, M
AU  - Quezada M
FAU - Lecocq, C
AU  - Lecocq C
FAU - Sierra, M A
AU  - Sierra MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein E2, classical swine fever virus)
SB  - IM
MH  - Animals
MH  - Brain/pathology/virology
MH  - Cattle
MH  - Classical Swine Fever/*pathology
MH  - Classical Swine Fever Virus/*isolation & purification
MH  - Diagnosis, Differential
MH  - Digestive System/pathology/virology
MH  - Immunohistochemistry/methods
MH  - Lymphoid Tissue/pathology/virology
MH  - Reference Values
MH  - Respiratory System/pathology/virology
MH  - Skin/pathology/virology
MH  - Swine
MH  - Urinary Tract/pathology/virology
MH  - Viral Envelope Proteins/*analysis
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1177/104063879700900103 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 1997 Jan;9(1):10-6. doi: 10.1177/104063879700900103.