PMID- 9088644
OWN - NLM
STAT- MEDLINE
DCOM- 19970612
LR  - 20191101
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 5
IP  - 1
DP  - 1997 Feb
TI  - Isolation and characterization of X chromosome-derived DNA sequences from a 
      dioecious plant Melandrium album.
PG  - 57-65
AB  - A number of X chromosome DNA sequences have been isolated from a dioecious plant, 
      Melandrium album (syn. Silene latifolia), using chromosome microdissection 
      followed by degenerate oligonucleotideprimed polymerase chain reaction (DOP-PCR) 
      amplification. Six DNA clones were selected and further characterized by DNA/DNA 
      hybridization techniques to check their copy numbers, sex-specific methylation 
      patterns, species specificity and positions on chromosomes. These clones were 
      moderately to highly repetitive (approximately 10(3)-10(5) copies per haploid 
      genome) and none of them gave a positive signal on Northern blots. One of the 
      clones yielded a sex-specific methylation pattern: its abundant non-methylated 
      CCGG island was found only in males. All the clones also hybridized to two 
      closely related dioecious Melandrium species (M. rubrum and M. dicline). 
      Nucleotide sequences of two X-derived clones showed a number of internal short 
      direct repeats; one of them strikingly resembled a plant conservative telomere 
      sequence (TTTAGGG). None of the clones hybridized to the X chromosome only, but 
      all were localized at the telomeric heterochromatic regions (DAPI C-bands) of 
      both arms of a vast majority of M. album chromosomes using the fluorescence in 
      situ hybridization (FISH) technique. However, the non-homologous arm of the Y 
      chromosome (contrary to the arm homologous to the X chromosome, possessing the 
      pseudoautosomal region) showed neither a DAPI C-banding-stained heterochromatin 
      nor a FISH signal with any of the DNA probes tested, thus indicating its 
      evolutionary diversification.
FAU - Buzek, J
AU  - Buzek J
AD  - Institute of Genetics and Molecular and Cellular Biology, Strasbourg, France.
FAU - Koutnikova, H
AU  - Koutnikova H
FAU - Houben, A
AU  - Houben A
FAU - Riha, K
AU  - Riha K
FAU - Janousek, B
AU  - Janousek B
FAU - Siroky, J
AU  - Siroky J
FAU - Grant, S
AU  - Grant S
FAU - Vyskot, B
AU  - Vyskot B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Plant)
RN  - 0 (Heterochromatin)
RN  - 0 (RNA, Plant)
SB  - IM
MH  - Base Composition
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Blotting, Southern
MH  - Chromosome Banding
MH  - Cloning, Molecular
MH  - *DNA Methylation
MH  - DNA Probes
MH  - *DNA, Plant/genetics/isolation & purification
MH  - Heterochromatin/ultrastructure
MH  - In Situ Hybridization, Fluorescence
MH  - Meiosis/genetics
MH  - Mitosis/genetics
MH  - Molecular Sequence Data
MH  - Plants/*genetics
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Genetic
MH  - RNA, Plant/genetics/isolation & purification
MH  - Repetitive Sequences, Nucleic Acid
MH  - Sequence Analysis, DNA
MH  - Sex Chromatin
MH  - Species Specificity
MH  - X Chromosome/*genetics/ultrastructure
MH  - Y Chromosome/genetics/ultrastructure
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - 10.1023/a:1011693603279 [doi]
PST - ppublish
SO  - Chromosome Res. 1997 Feb;5(1):57-65. doi: 10.1023/a:1011693603279.