PMID- 9089777
OWN - NLM
STAT- MEDLINE
DCOM- 19970610
LR  - 20190920
IS  - 0143-4004 (Print)
IS  - 0143-4004 (Linking)
VI  - 18
IP  - 2-3
DP  - 1997 Mar-Apr
TI  - The low proliferation rates of human amniotic cells are neither associated to 
      deregulated proto-oncogenes' expression nor to the effect of IFN alpha 2.
PG  - 163-8
AB  - Primary cultures of human amniotic membrane (PCHAM) cells display very low 
      proliferation rates while their doubling times vary between 150 h and 210 h even 
      after mitogenic stimuli. However, the pattern of proto-oncogenes (c-fos, c-myc 
      and c-jun) expression in these cells, upon serum restimulation, resembled that of 
      cell lines that display shorter population doubling times. Serum stimulation of 
      quiescent PCHAM cells promoted a rapid and transient c-fos mRNA expression, which 
      was detected within 10 min, reached maximal levels at 30 min and decreased to 
      undetectable levels 2-3 h later. The levels of c-myc or c-jun mRNA increased 
      within 10 min after serum restimulation, peaked at 3 h and decreased to 
      intermediate levels thereafter. We also present evidence showing that IFN alpha 2 
      treatment of PCHAM cells had no effect on their population doubling times nor in 
      c-fas, c-myc, or c-jun mRNA expression, under conditions in which induction of 
      IFN-stimulated genes, such as 2'-5' oligo-adenylate synthetase (OAS) and 6-16 was 
      observed. We conclude that the growth constraints observed with this cells are 
      not directly associated with a negative cellular growth regulation exerted by IFN 
      alpha 2, nor due to a deregulated proto-oncogenes' expression.
FAU - Oliveira, J G
AU  - Oliveira JG
AD  - Departamento de Microbiologia, Instituto de Ciencias Biologicas, Universidade 
      Federal de Minas Gerais, Belo Horizonte, Brazil.
FAU - Kroon, E G
AU  - Kroon EG
FAU - Ferreira, P C
AU  - Ferreira PC
FAU - Bonjardim, C A
AU  - Bonjardim CA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
RN  - 0 (Interferon-alpha)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amnion/*cytology/metabolism
MH  - Blotting, Northern
MH  - Cell Division
MH  - Cells, Cultured
MH  - Female
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Interferon-alpha/*pharmacology
MH  - Pregnancy
MH  - *Proto-Oncogenes
MH  - RNA, Messenger/isolation & purification
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - S0143-4004(97)90088-6 [pii]
AID - 10.1016/s0143-4004(97)90088-6 [doi]
PST - ppublish
SO  - Placenta. 1997 Mar-Apr;18(2-3):163-8. doi: 10.1016/s0143-4004(97)90088-6.