PMID- 9090750
OWN - NLM
STAT- MEDLINE
DCOM- 19970610
LR  - 20151119
IS  - 1078-0297 (Print)
IS  - 1078-0297 (Linking)
VI  - 95
IP  - 2
DP  - 1997 Feb
TI  - Inhibitory effects of tetrandrine and hernandezine on Ca2+ mobilization in rat 
      glioma C6 cells.
PG  - 129-46
AB  - The effects of tetrandrine (TET), a Ca2+ antagonist of Chinese herbal origin, and 
      hernandezine (HER), a structural analogue of TET, on Ca2+ mobilization were 
      studied in rat glioma C6 cells. TET and HER alone did not affect the resting 
      cytoplasmic Ca2+ concentration ([Ca2+]i). TET and HER inhibited the peak and 
      sustained elevation of [Ca2+]i induced by bombesin and thapsigargin (TG), a 
      microsomal Ca2+ ATPase inhibitor, in a dose-dependent manner. The doses of TET or 
      HER needed to abolish the sustained and peak increase in [Ca2+]i induced by 
      bombesin and TG were 30 microM and 300 microM, respectively. TET and HER did not 
      increase inositol 1,4,5-trisphosphate (IP3) accumulation by themselves but 
      inhibited IP3 accumulation elevated by bombesin. In permeabilized C6 cells, the 
      addition of IP3 and TG released Ca2+ from intracellular stores. Pretreatment with 
      TET or HER abolished Ca2+ release from intracellular stores induced by bombesin 
      and TG. In the absence of extracellular Ca2+, the addition of 3 mM Ca2+ to 
      extracellular medium slightly increased [Ca2+]i, which indicated Ca2+ entry due 
      to leakage of Ca2+ at the plasma membrane but not Ca2+ influx through Ca2+ 
      channels. TET and HER did not affect this leakage entry of Ca2+. The present 
      results suggest that TET and HER inhibit Ca2+ release from intracellular stores 
      as well as Ca2+ entry from extracellular medium evoked by bombesin and TG. In 
      addition, TET and HER inhibit IP3 accumulation induced by bombesin in rat glioma 
      C6 cells.
FAU - Imoto, K
AU  - Imoto K
AD  - Department of Pharmacology, School of Medicine, Sapporo Medical University, 
      Japan.
FAU - Takemura, H
AU  - Takemura H
FAU - Kwan, C Y
AU  - Kwan CY
FAU - Sakano, S
AU  - Sakano S
FAU - Kaneko, M
AU  - Kaneko M
FAU - Ohshika, H
AU  - Ohshika H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Res Commun Mol Pathol Pharmacol
JT  - Research communications in molecular pathology and pharmacology
JID - 9437512
RN  - 0 (Alkaloids)
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Benzylisoquinolines)
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Enzyme Inhibitors)
RN  - 29EX23D5AJ (tetrandrine)
RN  - 6681-13-6 (hernandezine)
RN  - 67526-95-8 (Thapsigargin)
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - PX9AZU7QPK (Bombesin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Alkaloids/*pharmacology
MH  - Animals
MH  - Antineoplastic Agents, Phytogenic/*pharmacology
MH  - *Benzylisoquinolines
MH  - Bombesin/toxicity
MH  - Calcium/*metabolism
MH  - Calcium Channel Blockers/*pharmacology
MH  - Cell Division/drug effects
MH  - Cell Membrane/drug effects/metabolism
MH  - Cell Membrane Permeability/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Enzyme Inhibitors/toxicity
MH  - Glioma/*metabolism/pathology
MH  - Inositol 1,4,5-Trisphosphate/metabolism
MH  - Rats
MH  - Thapsigargin/toxicity
MH  - Tumor Cells, Cultured
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
PST - ppublish
SO  - Res Commun Mol Pathol Pharmacol. 1997 Feb;95(2):129-46.