PMID- 9093942
OWN - NLM
STAT- MEDLINE
DCOM- 19970620
LR  - 20061115
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 51
IP  - 4
DP  - 1997 Apr
TI  - Amplification of GB virus-C/hepatitis G virus RNA with primers from different 
      regions of the viral genome.
PG  - 284-9
AB  - GB virus-C/hepatitis G virus (GBV-C/HGV) is a newly identified RNA virus. The aim 
      of the study was to compare three primer pairs from the 5' untranslated region 
      (5'UTR), envelope region 2 (E 2) and nonstructural region 3 (NS 3) of GBV-C/HGV 
      genome for their ability to detect GBV-C/HGV RNA by polymerase chain reaction 
      (PCR) assays. By using PCR with primers from different regions of the viral 
      genome, serum GBV-C/HGV RNA was assayed in 200 at-risk individuals. The 
      sensitivity of this assay was assessed by a titration experiment, and nucleotide 
      sequences of the amplified products were determined directly. Of 200 serum 
      samples, 43 (21.5%) were positive for GBV-C/HGV RNA with at least one of the 
      primer pairs. The positive rates by 5'UTR, NS 3, and E 2 primers were 100%, 98%, 
      and 84%, respectively, and the sensitivity of PCR assays using 5'UTR primers was 
      10 to 100 times more likely to detect GBV-C/HGV RNA than that of NS 3 and E 2 
      primers. The average homology of amplified targets to the prototype HGV genome 
      was 89%, 80%, and 85% and the similarity between each amplified target was up to 
      100%, 90%, and 92% in the 5'UTR, E 2, and NS 3 regions, respectively. Therefore, 
      the 5'UTR of GBV-C/HGV genome is highly conserved and primers deduced from this 
      region can provideva sensitive and specific PCR assay for GBV-C/HGV RNA.
FAU - Kao, J H
AU  - Kao JH
AD  - Department of Internal Medicine, National Taiwan University Hospital, National 
      Taiwan University College of Medicine, Taipei.
FAU - Chen, P J
AU  - Chen PJ
FAU - Chen, W
AU  - Chen W
FAU - Hsiang, S C
AU  - Hsiang SC
FAU - Lai, M Y
AU  - Lai MY
FAU - Chen, D S
AU  - Chen DS
LA  - eng
SI  - GENBANK/AF079474
SI  - GENBANK/AF079475
SI  - GENBANK/AF079476
SI  - GENBANK/AF079477
SI  - GENBANK/AF079478
SI  - GENBANK/AF079479
SI  - GENBANK/AF079480
SI  - GENBANK/AF079481
SI  - GENBANK/AF079482
SI  - GENBANK/AF079483
SI  - GENBANK/AF079854
SI  - GENBANK/AF079855
SI  - GENBANK/AF079856
SI  - GENBANK/AF079857
SI  - GENBANK/AF079858
SI  - GENBANK/AF079859
SI  - GENBANK/AF079860
SI  - GENBANK/AF079861
SI  - GENBANK/AF079862
SI  - GENBANK/AF079863
SI  - GENBANK/AF080107
SI  - GENBANK/AF080108
SI  - GENBANK/AF080109
SI  - GENBANK/AF080110
SI  - GENBANK/AF080111
SI  - GENBANK/AF080112
SI  - GENBANK/AF080113
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Base Sequence
MH  - DNA Primers
MH  - DNA, Viral
MH  - Flaviviridae/*genetics
MH  - *Genome, Viral
MH  - Hepatitis, Viral, Human/*virology
MH  - Humans
MH  - Molecular Sequence Data
MH  - *Polymerase Chain Reaction
MH  - *RNA, Viral
MH  - Sensitivity and Specificity
EDAT- 1997/04/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1002/(SICI)1096-9071(199704)51:4<284::AID-JMV5>3.0.CO;2-1 [pii]
PST - ppublish
SO  - J Med Virol. 1997 Apr;51(4):284-9.