PMID- 9094650
OWN - NLM
STAT- MEDLINE
DCOM- 19970425
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 71
IP  - 5
DP  - 1997 May
TI  - Induction of T-cell apoptosis by human herpesvirus 6.
PG  - 3751-9
AB  - The mechanisms of cell death in CD4+ T cells mediated by human herpesvirus 6 
      (HHV-6) were investigated. The frequency of cell death in the human CD4+ T-cell 
      line JJHAN, which had been inoculated with HHV-6 variant A or B, appeared to be 
      augmented by tumor necrosis factor alpha (TNF-alpha). Agarose gel electrophoresis 
      of DNA from HHV-6-inoculated cells showed DNA fragmentation in multiples of the 
      oligonucleosome length unit. The degree of DNA fragmentation increased when 
      HHV-6-inoculated cells were cultured in the presence of TNF-alpha. Flow cytometry 
      and Scatchard analysis of TNF receptors revealed an increase in the number of the 
      p55 form of TNF receptors on JJHAN cells after HHV-6 inoculation. It also 
      appeared that treatment with anti-Fas monoclonal antibody (MAb) induced marked 
      apoptosis in HHV-6-inoculated cells. Transmission electron microscopy showed 
      characteristics of apoptosis, such as chromatin condensation and fragmentation of 
      nuclei, but virus particles were hardly detected in apoptotic cells. Two-color 
      flow cytometric analysis using anti-HHV-6 MAb and propidium iodide revealed that 
      DNA fragmentation was present predominantly in uninfected cells but not in 
      productively HHV-6-infected cells. In addition, JJHAN cells incubated with UV 
      light-irradiated and ultracentrifuged culture supernatant of HHV-6-infected cells 
      appeared to undergo apoptosis. The present study demonstrated that both HHV-6 
      variants A and B induce apoptosis in CD4+ T cells by indirect mechanisms, as 
      reported recently in human immunodeficiency virus type 1 infection.
FAU - Inoue, Y
AU  - Inoue Y
AD  - First Department of Internal Medicine, Ehime University School of Medicine, 
      Shigenobu, Japan.
FAU - Yasukawa, M
AU  - Yasukawa M
FAU - Fujita, S
AU  - Fujita S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (fas Receptor)
SB  - IM
MH  - *Apoptosis
MH  - CD4-Positive T-Lymphocytes/pathology/*virology
MH  - Cells, Cultured
MH  - DNA Fragmentation
MH  - Flow Cytometry
MH  - Herpesvirus 6, Human/*physiology
MH  - Humans
MH  - Proto-Oncogene Proteins c-bcl-2/analysis
MH  - Receptors, Tumor Necrosis Factor/analysis
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - fas Receptor/physiology
PMC - PMC191525
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
PMCR- 1997/05/01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
PHST- 1997/05/01 00:00 [pmc-release]
AID - 10.1128/JVI.71.5.3751-3759.1997 [doi]
PST - ppublish
SO  - J Virol. 1997 May;71(5):3751-9. doi: 10.1128/JVI.71.5.3751-3759.1997.