PMID- 9096064
OWN - NLM
STAT- MEDLINE
DCOM- 19970612
LR  - 20191210
IS  - 0022-2631 (Print)
IS  - 0022-2631 (Linking)
VI  - 156
IP  - 3
DP  - 1997 Apr 1
TI  - Characterization of ryanodine-sensitive Ca2+ release from microsomal vesicles of 
      rat parotid acinar cells: regulation by cyclic ADP-ribose.
PG  - 231-9
AB  - We have measured ryanodine (caffeine)-sensitive 45Ca2+ release from isolated 
      microsomal vesicles of endoplasmic reticulum prepared from rat parotid acinar 
      cells. After a steady state of ATP-dependent 45Ca2+ uptake, the addition of 
      caffeine (40 mm), ryanodine (10 approximately 500 microm) or an NAD+ metabolite, 
      cyclic ADP-ribose (cADPR, 4 microm) released about 10% of the 45Ca2+ that had 
      been taken up. The 45Ca2+ release was not inhibited by heparin, an antagonist of 
      IP3 receptor. The effects of caffeine, ryanodine and cADPR on 45Ca2+ release were 
      also tested in the presence of thapsigargin (TG), an inhibitor of microsomal 
      Ca2+-ATPase. When caffeine (10 approximately 40 mm), ryanodine (10 microm) or 
      cADPR (1 approximately 10 microm) was added in the medium with 100 nm TG, a 
      significant 45Ca2+ release was seen, while higher concentrations of ryanodine 
      (>100 microm) did not cause any 45Ca2+ release in the presence of TG. The initial 
      rate of caffeine (40 mm)-induced 45Ca2+ release was increased by a pretreatment 
      with 10 microm ryanodine, whereas the caffeine-induced 45Ca2+ release was 
      strongly inhibited by the presence of a higher concentration (500 microm) of 
      ryanodine. cADPR-induced 45Ca2+ release was also inhibited by 500 microm 
      ryanodine. Caffeine (40 mm)- or cADPR (4 microm)-induced 45Ca2+ release was 
      abolished by a presence of ruthenium red (50 approximately 100 microm). The 
      presence of a low concentration (0.5 microm) of cADPR shifted the dose-response 
      curve of caffeine-induced 45Ca2+ release to the left. These results indicate the 
      presence of a ryanodine sensitive Ca2+ release mechanism in the endoplasmic 
      reticulum of rat parotid acinar cells that is distinct from the IP3-sensitive 
      Ca2+ channel and is activated by caffeine, cADPR and a low concentration (10 
      microm) of ryanodine, but is inhibited by higher concentrations (>100 microm) of 
      ryanodine and ruthenium red. The properties of the ryanodine-sensitive mechanism 
      are similar to that of the ryanodine receptor as described in muscle cells.
FAU - Ozawa, T
AU  - Ozawa T
AD  - Department of Physiology, Tohoku University School of Medicine, Seiryocho 2, 
      980-77, Sendai, Japan.
FAU - Nishiyama, A
AU  - Nishiyama A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Membr Biol
JT  - The Journal of membrane biology
JID - 0211301
RN  - 0 (Calcium Channels)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Inositol 1,4,5-Trisphosphate Receptors)
RN  - 0 (Muscle Proteins)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Ryanodine Receptor Calcium Release Channel)
RN  - 11103-72-3 (Ruthenium Red)
RN  - 119340-53-3 (Cyclic ADP-Ribose)
RN  - 15662-33-6 (Ryanodine)
RN  - 20762-30-5 (Adenosine Diphosphate Ribose)
RN  - 3G6A5W338E (Caffeine)
RN  - 67526-95-8 (Thapsigargin)
RN  - 9005-49-6 (Heparin)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adenosine Diphosphate Ribose/*analogs & derivatives/physiology
MH  - Animals
MH  - Caffeine/pharmacology
MH  - Calcium/*metabolism
MH  - Calcium Channels/drug effects/*metabolism
MH  - Calcium-Transporting ATPases/antagonists & inhibitors
MH  - Cells, Cultured
MH  - Cyclic ADP-Ribose
MH  - Dose-Response Relationship, Drug
MH  - Endoplasmic Reticulum/drug effects/*metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Heparin/pharmacology
MH  - Inositol 1,4,5-Trisphosphate Receptors
MH  - Male
MH  - Microsomes/drug effects/*metabolism
MH  - Muscle Proteins/drug effects/*metabolism
MH  - Parotid Gland/cytology/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Cytoplasmic and Nuclear/antagonists & inhibitors
MH  - Ruthenium Red/pharmacology
MH  - Ryanodine/pharmacology
MH  - Ryanodine Receptor Calcium Release Channel
MH  - Thapsigargin/pharmacology
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1007/s002329900203 [doi]
PST - ppublish
SO  - J Membr Biol. 1997 Apr 1;156(3):231-9. doi: 10.1007/s002329900203.