PMID- 9096316
OWN - NLM
STAT- MEDLINE
DCOM- 19970508
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 94
IP  - 7
DP  - 1997 Apr 1
TI  - Nucleosome-mediated synergism between transcription factors on the mouse mammary 
      tumor virus promoter.
PG  - 2885-90
AB  - In unstimulated mammalian cells and in Saccharomyces cerevisiae, the mouse 
      mammary tumor virus (MMTV) promoter is silent and organized into positioned 
      nucleosomes, one of which encompasses the binding sites for glucocorticoid 
      receptor (GR) and nuclear factor I (NFI). Glucocorticoid induction in vivo 
      involves a functional synergism between GR and NFI and simultaneous occupancy of 
      the promoter sites for both proteins that cannot be reproduced on naked DNA. The 
      role of chromatin in the process of induction was investigated by manipulating 
      the nucleosome density in yeast strains carrying a regulated histone H4 gene. 
      Following depletion of nucleosomes, independent transactivation by NFI or by GR, 
      as well as binding of the individual proteins to the MMTV promoter, were 
      enhanced, in agreement with a repressive function of nucleosomes. In contrast, 
      NFI-dependent hormone induction of the promoter and the simultaneous binding of 
      receptor and NFI were compromised by nucleosome depletion. This effect could be 
      partly mediated by a cryptic binding site for the receptor that is functional 
      only in the nucleosomal context. Thus, positioned nucleosomes do not only account 
      for constitutive repression of the MMTV promoter, but also participate in 
      induction by mediating cooperative binding and functional synergism between GR 
      and NFI.
FAU - Chavez, S
AU  - Chavez S
AD  - Institut fur Molekularbiologie und Tumorforschung, Marburg, Germany.
FAU - Beato, M
AU  - Beato M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (NFI Transcription Factors)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nucleosomes)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Transcription Factors)
RN  - 0 (Y-Box-Binding Protein 1)
RN  - 0 (YBX1 protein, human)
SB  - IM
MH  - *CCAAT-Enhancer-Binding Proteins
MH  - DNA Footprinting
MH  - DNA-Binding Proteins/metabolism
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - NFI Transcription Factors
MH  - Nuclear Proteins
MH  - Nucleosomes/*metabolism
MH  - *Promoter Regions, Genetic
MH  - Receptors, Glucocorticoid/metabolism
MH  - Transcription Factors/*metabolism
MH  - Transcriptional Activation
MH  - Y-Box-Binding Protein 1
PMC - PMC20292
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
PMCR- 1997/10/01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
PHST- 1997/10/01 00:00 [pmc-release]
AID - 2704 [pii]
AID - 10.1073/pnas.94.7.2885 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):2885-90. doi: 10.1073/pnas.94.7.2885.