PMID- 9096351
OWN - NLM
STAT- MEDLINE
DCOM- 19970508
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 94
IP  - 7
DP  - 1997 Apr 1
TI  - The winged helix transcription factor HFH-4 is expressed during choroid plexus 
      epithelial development in the mouse embryo.
PG  - 3094-9
AB  - Mammalian hepatocyte nuclear factor-3 (HNF-3) and the Drosophila homeotic gene 
      fork head proteins are prototypes of an extensive family of cell-specific 
      transcription factors that share homology in the winged helix DNA-binding domain. 
      One of these mammalian family members, HNF-3/fork head homolog-4 (HFH-4), was 
      isolated by PCR amplification of rodent brain cDNA and exhibits abundant 
      expression in the adult bronchiolar epithelium. In this study, we performed in 
      situ hybridization of stage-specific mouse embryos and report on a novel 
      expression pattern of the HFH-4 gene in both the presumptive and differentiated 
      choroid plexus epithelium, which is responsible for the synthesis and secretion 
      of cerebrospinal fluid (CSF) proteins. We also showed that HFH-4 is a potent 
      transcriptional activator in cotransfection assays and defined several protein 
      sequences important for HFH-4 transcriptional activity. We used in vitro 
      DNA-binding site selection with recombinant HFH-4 protein and determined that the 
      HFH-4 protein recognizes the DNA consensus sequences HWDTGTTTGTTTA or 
      KTTTGTTGTTKTW (where H is not G, W is A or T, D is not C, and K is G or T). We 
      used this HFH-4 consensus to identify potential HFH-4 target genes in the choroid 
      plexus epithelium and demonstrated that these promoter sequences bind to 
      recombinant HFH-4 protein in electrophoretic mobility shift assays. Recombinant 
      HFH-4 formed specific protein-DNA complexes with the promoter regions of the 
      human prothrombin, beta amyloid precursor protein, alpha1-antichymotrypsin, 
      cystic fibrosis transmembrane conductance regulator and rodent 
      alpha2-macroglobulin, growth hormone receptors, and insulin-like growth factor II 
      genes. Furthermore, we identified putative HFH-4 target genes in the bronchiolar 
      epithelium including the clara cell secretory protein gene and the HNF-3alpha 
      gene, a winged helix family member involved in the transcriptional regulation of 
      genes in the bronchiolar epithelium. In support of these binding studies, 
      cotransfection assays show that HFH-4 potentiates expression of the HNF-3alpha 
      and clara cell secretory protein promoter regions.
FAU - Lim, L
AU  - Lim L
AD  - University of Illinois at Chicago, College of Medicine, Department of 
      Biochemistry, 60612-7334, USA.
FAU - Zhou, H
AU  - Zhou H
FAU - Costa, R H
AU  - Costa RH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bronchi/embryology/metabolism
MH  - Choroid Plexus/embryology/*metabolism
MH  - DNA-Binding Proteins/genetics
MH  - Epithelium/embryology/metabolism
MH  - *Gene Expression Regulation, Developmental
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Transcription Factors/*genetics
PMC - PMC20327
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
PMCR- 1997/10/01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
PHST- 1997/10/01 00:00 [pmc-release]
AID - 2612 [pii]
AID - 10.1073/pnas.94.7.3094 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3094-9. doi: 10.1073/pnas.94.7.3094.