PMID- 9096692
OWN - NLM
STAT- MEDLINE
DCOM- 19970429
LR  - 20190914
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 11
IP  - 4
DP  - 1997 Apr
TI  - Mosaicism of the 5q deletion as assessed by interphase FISH is a common 
      phenomenon in MDS and restricted to myeloid cells.
PG  - 519-23
AB  - Interstitial deletion of chromosome 5q is a common cytogenetic abnormality 
      observed in MDS. We have used fluorescence in situ hybridization (FISH) to 
      determine accurately the percentage of cytogenetically abnormal peripheral blood 
      cells. YAC and cosmid probes localized to chromosome 5q were hybridized to 
      interphase nuclei from purified polymorphonuclear cells (PMNs) from six MDS 
      patients with chromosome 5 deletions. Per patient, 25-67% of the cells exhibited 
      one signal for the 5q31-q33 specific probes IL-4, D5S207 and c-fms. This 
      percentage was constant for the various probes utilized for each patient. 
      Hybridization of the same probes to PMNs from healthy individuals and 
      hybridization of probes (D5S39 and D5S498) localized outside the deleted segments 
      to PMNs of the patients, resulted in 90-95% nuclei with two signals. In addition, 
      FACS-purified peripheral blood cells were investigated by FISH using the IL-4 
      cosmid. This demonstrated that the hybridization pattern in monocytes was similar 
      to that observed in PMNs, whereas T-lymphocytes showed no loss of signals. These 
      results indicate that a subfraction of the myeloid progenitor cells have acquired 
      the 5q deletion.
FAU - Kroef, M J
AU  - Kroef MJ
AD  - Laboratory for Experimental Haematology, Leiden University Hospital, The 
      Netherlands.
FAU - Bolk, M J
AU  - Bolk MJ
FAU - Muus, P
AU  - Muus P
FAU - Wessels, J W
AU  - Wessels JW
FAU - Beverstock, G C
AU  - Beverstock GC
FAU - Willemze, R
AU  - Willemze R
FAU - Landegent, J E
AU  - Landegent JE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
SB  - IM
MH  - Adult
MH  - Bone Marrow/ultrastructure
MH  - Cells, Cultured
MH  - *Chromosomes, Human, Pair 5
MH  - *Gene Deletion
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics/pathology
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1038/sj.leu.2400597 [doi]
PST - ppublish
SO  - Leukemia. 1997 Apr;11(4):519-23. doi: 10.1038/sj.leu.2400597.