PMID- 9096702
OWN - NLM
STAT- MEDLINE
DCOM- 19970429
LR  - 20190914
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 11
IP  - 4
DP  - 1997 Apr
TI  - Establishment of a novel human acute myeloblastic leukemia cell line (YNH-1) with 
      t(16;21), t(1;16) and 12q13 translocations.
PG  - 599-608
AB  - The t(16;21)(p11;q22) translocation is a non-random chromosomal aberration 
      observed in several types of human acute myeloblastic leukemia (AML), whereas the 
      der(16)t(1;16) and chromosome rearrangements at 12q13 are frequently found in 
      solid tumors. A novel cell line YNH-1 was established from peripheral blood cells 
      of a 46-year-old male with AML (M1) carrying t(16;21) and t(1;16) translocations. 
      YNH-1 has been maintained with a doubling time of 82 h for more than 20 months as 
      a granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage 
      colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) dependent line. 
      Morphologically YNH-1 cells were free-floating immature myeloblasts with 
      lobulated nuclei and vacuoles in the cytoplasm. They were positive for 
      myeloperoxidase but negative for alpha-naphthyl butylate esterase and 
      chloroacetate esterase stainings. In surface marker analysis YNH-1 cells were 
      positive for CD13, CD33 and CD34. Chromosomal analysis showed 46, XY, 
      der(16)t(16;21)(p11;q22)t(1;16) (q12;q13), der(21)t(16;21)(p11;q22), der 
      (6)t(6;12)(q13;q13), der(12)t(6;12)(q21;q13). These translocations were confirmed 
      by fluorescence in situ hybridization (FISH) studies with the ERG-YAC clone and 
      chromosome-specific DNA libraries. Both the FUS/ERG and ERG/FUS chimeric 
      transcripts were identified by reverse transcriptase-polymerase chain reaction 
      (RT-PCR) analysis. Thus, YNH-1 could be a useful tool for elucidating the 
      pathophysiology and molecular mechanism in AML with t(16;21),t(1;16) and 12q13 
      translocations.
FAU - Yamamoto, K
AU  - Yamamoto K
AD  - Department of Hematology, Musashino Red Cross Hospital, Tokyo, Japan.
FAU - Hamaguchi, H
AU  - Hamaguchi H
FAU - Nagata, K
AU  - Nagata K
FAU - Kobayashi, M
AU  - Kobayashi M
FAU - Tanimoto, F
AU  - Tanimoto F
FAU - Taniwaki, M
AU  - Taniwaki M
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Interleukin-3)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Chromosomes, Human, Pair 1
MH  - *Chromosomes, Human, Pair 16
MH  - *Chromosomes, Human, Pair 21
MH  - Granulocyte Colony-Stimulating Factor/pharmacology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interleukin-3/pharmacology
MH  - Karyotyping
MH  - Leukemia, Myeloid, Acute/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - *Translocation, Genetic
MH  - Tumor Cells, Cultured/drug effects
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1038/sj.leu.2400594 [doi]
PST - ppublish
SO  - Leukemia. 1997 Apr;11(4):599-608. doi: 10.1038/sj.leu.2400594.