PMID- 9098576
OWN - NLM
STAT- MEDLINE
DCOM- 19970609
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 751
IP  - 1
DP  - 1997 Mar 14
TI  - Androgen inhibits neurotransmitter turnover in the medial prefrontal cortex of 
      the rat following exposure to a novel environment.
PG  - 131-8
AB  - Previous studies have demonstrated that gonadal steroid hormones affect the 
      neuroendocrine response to a novel environment and other stressors. Introduction 
      to a novel environment also increases neurotransmitter turnover in the medial 
      prefrontal cortex (MPFC). In this study, we examined the possibility that gonadal 
      steroid hormones could similarly modulate the neurotransmitter response to a 
      novel environment in the MPFC of the male rat. Male Fischer 344 rats at 3 months 
      of age were gonadectomized (GDX'd) and implanted with Silastic capsules 
      containing dihydrotestosterone propionate (DHTP, a non-aromatizable form of 
      androgen), 17 beta-estradiol (E), or placebo. Control animals were left intact. 
      Each of these groups was further divided into a group introduced to a novel 
      environment or a home cage control group. Animals exposed to a novel environment 
      were killed after spending 20 min in a novel open field, whereas control animals 
      were killed immediately upon removal from their home cage. Using high performance 
      liquid chromatography, the MPFC was assayed for tissue levels of dopamine (DA) 
      and its metabolites, 3,4-dihydroxyphenylalanine (DOPAC) and homovanillic acid 
      (HVA); norepinephrine (NE) and its metabolite 3-methoxy-4-hydroxyphenylglycol 
      (MHPG); or serotonin (5-HT) and its metabolite 5-hydroxyindole acetic acid 
      (5-HIAA). The introduction to a novel environment caused significant increases in 
      turnover of all three neurochemicals examined as estimated by 
      metabolite/precursor ratios. These increases were characterized by increases in 
      DOPAC, HVA, MHPG and 5-HIAA coupled with decreases in DA, NE and 5-HT. There was 
      no effect of GDX on neurotransmitter turnover, however, treatment of GDX animals 
      with DHTP prevented the open field induced increase in DOPAC/DA, MHPG/NE, and 
      5-HIAA/5-HT ratio. Treatment of GDX animals with estrogen had the opposite effect 
      of DHTP, DOPAC/DA and MHPG/NE ratios increased to a greater level following the 
      introduction to a novel environment than in GDX or intact animals. Examination of 
      behavior in the open field showed significant decreases in activity in the 
      DHTP-treated group but not in any other behavioral parameter (rears, nose pokes). 
      Since the non-aromatizable androgen, DHTP, is presumably acting via androgen 
      receptors, and E is presumably acting via estrogen receptors, these data suggest 
      that, in the MPFC of male rats, androgen and estrogen receptors act in an 
      opposing fashion to modify neurotransmitter turnover. This suggests that local 
      changes in the relative levels of androgen and estrogen can have profound effects 
      on the neurobiological response of the medial prefrontal cortex to stimuli.
FAU - Handa, R J
AU  - Handa RJ
AD  - Department of Cell Biology, Loyola University, Chicago, Stritch School of 
      Medicine, Maywood, IL 60153, USA. rhanda@wpo.it.luc.edu
FAU - Hejna, G M
AU  - Hejna GM
FAU - Lorens, S A
AU  - Lorens SA
LA  - eng
GR  - AA08696/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Neurotransmitter Agents)
RN  - 08J2K08A3Y (Dihydrotestosterone)
RN  - 102-32-9 (3,4-Dihydroxyphenylacetic Acid)
RN  - 333DO1RDJY (Serotonin)
RN  - 534-82-7 (Methoxyhydroxyphenylglycol)
RN  - 54-16-0 (Hydroxyindoleacetic Acid)
RN  - 63-84-3 (Dihydroxyphenylalanine)
RN  - VTD58H1Z2X (Dopamine)
RN  - X4W3ENH1CV (Norepinephrine)
RN  - X77S6GMS36 (Homovanillic Acid)
SB  - IM
MH  - 3,4-Dihydroxyphenylacetic Acid/metabolism
MH  - Animals
MH  - Behavior, Animal/physiology
MH  - Chromatography, High Pressure Liquid
MH  - Dihydrotestosterone/*pharmacology
MH  - Dihydroxyphenylalanine/metabolism
MH  - Dopamine/metabolism
MH  - Exploratory Behavior/*physiology
MH  - Homovanillic Acid/metabolism
MH  - Hydroxyindoleacetic Acid/metabolism
MH  - Male
MH  - Methoxyhydroxyphenylglycol/metabolism
MH  - Neurotransmitter Agents/*metabolism
MH  - Norepinephrine/metabolism
MH  - Prefrontal Cortex/drug effects/*metabolism
MH  - Rats
MH  - Rats, Inbred F344
MH  - Serotonin/metabolism
EDAT- 1997/03/14 00:00
MHDA- 1997/03/14 00:01
CRDT- 1997/03/14 00:00
PHST- 1997/03/14 00:00 [pubmed]
PHST- 1997/03/14 00:01 [medline]
PHST- 1997/03/14 00:00 [entrez]
AID - S0006-8993(96)01394-7 [pii]
AID - 10.1016/s0006-8993(96)01394-7 [doi]
PST - ppublish
SO  - Brain Res. 1997 Mar 14;751(1):131-8. doi: 10.1016/s0006-8993(96)01394-7.