PMID- 9098720
OWN - NLM
STAT- MEDLINE
DCOM- 19970619
LR  - 20031114
IS  - 1023-3830 (Print)
IS  - 1023-3830 (Linking)
VI  - 46
IP  - 3
DP  - 1997 Mar
TI  - Effect of slow release IL-12 and IL-10 on inflammation, local macrophage function 
      and the regional lymphoid response during mycobacterial (Th1) and schistosomal 
      (Th2) antigen-elicited pulmonary granuloma formation.
PG  - 86-92
AB  - OBJECTIVE AND DESIGN: This study examines the local and regional effects of 
      exogenously administered interleukins 10 (IL-10) and 12 (IL-12) on pulmonary 
      granulomas mediated by Th1/type 1-(IFN-gamma) and Th2/type 2-(IL-4, IL-5 
      cytokines. MATERIALS AND TREATMENTS: Granulomas (GR) were induced in 
      presensitized CBA mice by embolization of beads coated with Mycobacteria 
      tuberculosis or Schistosoma mansoni egg antigens. Before challenge, osmotic pumps 
      distributing IL-10 or IL-12 (50 micrograms/kg/day) were implanted 
      intraperitoneally, then GR and draining lymph nodes were examined 4 days. 
      METHODS: GR sizes and composition were determined by morphometry and differential 
      analysis. Isolated GR macrophages and draining lymph nodes were assessed for 
      cytokine production by ELISA. RESULTS: IL-10 did not effect GR sizes but reduced 
      neutrophils in type 1 GR. IL-12 minimally reduced type 1 GR but decreased the 
      type 2 lesion by up to 70%, primarily curtailing eosinophils. Type 2 GR 
      macrophages were unaffected but type 1 were impaired by IL-10. Conversely, type 
      GR macrophages were more resistant to IL-12 while type 2 showed enhanced IL-10, 
      IL-12 and TNF, but reduced MCP-I production. In lymph nodes, IL-10 caused 
      paradoxical effects, enhancing IFN-gamma in the type 1 and decreasing Th2 
      cytokines in the type 2 response. Exogenous IL-12 profoundly augmented IFN-gamma 
      and abrogated type 2 cytokines while inhibiting intrinsic IL-12 production in 
      lymph nodes. CONCLUSION: These findings provide novel information regarding 
      cytokine regulation and the effects of systemic cytokine therapy.
FAU - Chensue, S W
AU  - Chensue SW
AD  - Department of Pathology and Laboratory Medicine, Veterans Affairs Medical Center, 
      Ann Arbor, MI 48105, USA.
FAU - Warmington, K
AU  - Warmington K
FAU - Ruth, J H
AU  - Ruth JH
FAU - Kunkel, S L
AU  - Kunkel SL
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Inflamm Res
JT  - Inflammation research : official journal of the European Histamine Research 
      Society ... [et al.]
JID - 9508160
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Antigens, Helminth)
RN  - 0 (Delayed-Action Preparations)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - Antigens, Bacterial/immunology
MH  - Antigens, Helminth/immunology
MH  - Delayed-Action Preparations
MH  - Female
MH  - Granuloma/*drug therapy/immunology/pathology
MH  - Inflammation/*drug therapy/immunology
MH  - Interleukin-10/*therapeutic use
MH  - Interleukin-12/*therapeutic use
MH  - Lung Diseases/*drug therapy/immunology/pathology
MH  - Lymph Nodes/drug effects/immunology
MH  - Macrophages, Alveolar/*drug effects/immunology
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Mycobacterium tuberculosis/immunology
MH  - Schistosoma mansoni/immunology
MH  - Th1 Cells/immunology
MH  - Th2 Cells/immunology
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - 10.1007/s000110050101 [pii]
AID - 10.1007/s000110050101 [doi]
PST - ppublish
SO  - Inflamm Res. 1997 Mar;46(3):86-92. doi: 10.1007/s000110050101.