PMID- 9099584 OWN - NLM STAT- MEDLINE DCOM- 19970731 LR - 20191101 IS - 0962-1075 (Print) IS - 0962-1075 (Linking) VI - 6 IP - 2 DP - 1997 May TI - FISH landmarks for Aedes aegypti chromosomes. PG - 197-202 AB - Aedes aegypti metaphase chromosome landmarks have been developed so that each chromosome of the haploid genome can be unambiguously identified and oriented by fluorescence in situ hybridization (FISH) and digital imaging microscopy. The FISH tags were derived from three cosmids that contain repetitive Ae. aegypti sequences and their unique FISH tagging characteristics are demonstrated. The sequence of the three chromosomal tags revealed that the chromosome 1 tag is an 18S fragment from the ribosomal cistron, and the other two chromosomal tags are repeats found in Ae. aegypti with no apparent similarity to known sequences. A single plasmid that contains the three chromosomes tag sequences has been constructed to simplify future FISH physical mapping. FAU - Brown, S E AU - Brown SE AD - Department of Bioagricultural Sciences and Pest Management, College of Agricultural Sciences, Colorado State University, Fort Collins 80523, USA. FAU - Knudson, D L AU - Knudson DL LA - eng SI - GENBANK/U65373 SI - GENBANK/U65374 SI - GENBANK/U65375 GR - AI34337/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - England TA - Insect Mol Biol JT - Insect molecular biology JID - 9303579 RN - 9007-49-2 (DNA) SB - IM MH - Aedes/*genetics MH - Animals MH - Base Sequence MH - Cell Line MH - Chromosomes MH - DNA MH - *In Situ Hybridization, Fluorescence MH - Molecular Sequence Data EDAT- 1997/05/01 00:00 MHDA- 1997/05/01 00:01 CRDT- 1997/05/01 00:00 PHST- 1997/05/01 00:00 [pubmed] PHST- 1997/05/01 00:01 [medline] PHST- 1997/05/01 00:00 [entrez] AID - 10.1111/j.1365-2583.1997.tb00088.x [doi] PST - ppublish SO - Insect Mol Biol. 1997 May;6(2):197-202. doi: 10.1111/j.1365-2583.1997.tb00088.x.