PMID- 9100135
OWN - NLM
STAT- MEDLINE
DCOM- 19970627
LR  - 20190905
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 380
IP  - 2
DP  - 1997 Apr 7
TI  - Prenatal ontogeny of the epidermal growth factor receptor and its ligand, 
      transforming growth factor alpha, in the rat brain.
PG  - 243-61
AB  - Transforming growth factor alpha (TGF alpha) interacts with the epidermal growth 
      factor receptor (EGF-R) to produce its biological effects. TGF alpha induces the 
      proliferation and differentiation of central nervous system (CNS) astrocytes and 
      pluripotent stem cells, as well as the survival and differentiation of 
      postmitotic CNS neurons. Both TGF alpha and EGF-R have been localized to the 
      postnatal CNS. As the majority of CNS neuronal proliferation and migration occurs 
      antenatally, we have examined the ontogeny of TGF alpha and EGF-R in the 
      embryonic rat brain by in situ hybridization. EGF-R mRNA was expressed in the 
      brain as early as embryonic day 11 (E11; the earliest age examined). It was 
      initially detected in the midbrain, with subsequent expression first in multiple 
      germinal zones, followed by expression in numerous cells throughout the brain. In 
      many brain areas, EGF-R mRNA appeared in germinal centers during the later stages 
      of neurogenesis and the early stages of gliogenesis. In the midbrain, the 
      distribution of EGF-R mRNA overlapped extensively with that of tyrosine 
      hydroxylase mRNA, suggesting that fetal dopaminergic neurons express EGF-R. 
      Immunocytochemistry was used to demonstrate the presence of EGF-R-immunoreactive 
      protein in brain areas that expressed EGF-R mRNA on E15 and E20. The expression 
      of TGF alpha in many brain structures preceded that of EGF-R mRNA. TGF alpha mRNA 
      was distributed throughout many non-germinal centers of the brain on E12 and 
      later. Some brain areas, such as the external granule cell layer of the 
      cerebellum, expressed EGF-R, but not TGF alpha mRNA. Northern blot analysis 
      demonstrated that mRNA species for both TGF alpha and EGF-R were similar in 
      embryos and adults. These data indicate that TGF alpha and EGF-R are positioned 
      to have a role in the genesis, differentiation, migration, or survival of 
      numerous cell populations in the embryonic brain.
FAU - Kornblum, H I
AU  - Kornblum HI
AD  - Department of Molecular and Medical Pharmacology, UCLA School of Medicine 90095, 
      USA. Harley@UCLA.Edu
FAU - Hussain, R J
AU  - Hussain RJ
FAU - Bronstein, J M
AU  - Bronstein JM
FAU - Gall, C M
AU  - Gall CM
FAU - Lee, D C
AU  - Lee DC
FAU - Seroogy, K B
AU  - Seroogy KB
LA  - eng
GR  - CA43793/CA/NCI NIH HHS/United States
GR  - NS 35164/NS/NINDS NIH HHS/United States
GR  - NS01837/NS/NINDS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Transforming Growth Factor alpha)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Animals
MH  - Brain/*metabolism
MH  - Embryo, Mammalian/*metabolism
MH  - Embryonic and Fetal Development
MH  - ErbB Receptors/*metabolism
MH  - Female
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transforming Growth Factor alpha/*metabolism
EDAT- 1997/04/07 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/04/07 00:00
PHST- 1997/04/07 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/04/07 00:00 [entrez]
AID - 10.1002/(SICI)1096-9861(19970407)380:2<243::AID-CNE7>3.0.CO;2-3 [pii]
AID - 10.1002/(sici)1096-9861(19970407)380:2<243::aid-cne7>3.0.co;2-3 [doi]
PST - ppublish
SO  - J Comp Neurol. 1997 Apr 7;380(2):243-61. doi: 
      10.1002/(sici)1096-9861(19970407)380:2<243::aid-cne7>3.0.co;2-3.