PMID- 9100993
OWN - NLM
STAT- MEDLINE
DCOM- 19970624
LR  - 20061115
IS  - 0889-2229 (Print)
IS  - 0889-2229 (Linking)
VI  - 13
IP  - 6
DP  - 1997 Apr 10
TI  - Transcription of human endogenous retroviral sequences related to mouse mammary 
      tumor virus in human breast and placenta: similar pattern in most malignant and 
      nonmalignant breast tissues.
PG  - 507-16
AB  - The human genome contains a large variety of sequences related to the mouse 
      mammary tumor virus (MMTV). We have investigated the range of expression of human 
      endogenous retroviral sequences (HERVs) related to MMTV (human MMTV-like; HML) as 
      RNA in 60 breast cancers, 8 nonmalignant breast tissues, and 9 placentas. This 
      was monitored using HML group-specific oligonucleotide probes in hybridizations 
      toward PCR amplificates of HML pol sequences and internal control. The degree of 
      expression of five HML groups varied between individuals and between tissues. On 
      average, all HML groups were less expressed in breast tissues than in placenta. 
      The hybridization signals of some HML RNAs were strongly correlated, indicating a 
      nonstochastic mechanism and a concerted regulation of their expression. The PCR 
      product from one breast cancer (BC 6), which gave an exceptionally high 
      expression with probe hml-6, with a 20 times stronger signal than the rest of the 
      cancers, was cloned and sequenced. The HML-6 transcript sequences were 
      homogeneous in BC 6. The most predominant clone derived from the cancer was used 
      as a probe in Southern hybridizations. The same restriction fragments were 
      detected in human breast tissues, PBMCs (peripheral blood mononuclear cells), and 
      breast cancer cell lines, except for one of the breast cancers and one of the 
      nonmalignant breast tissues, which gave different banding patterns. A comparison 
      of HML expression in normal and malignant breast tissue from the same individual 
      would have been more precise than our comparison of samples from different 
      persons. Bearing this limitation in mind, with a single exception, human 
      MMTV-like sequences were not more actively expressed in malignant than in 
      nonmalignant breast tissues. Nevertheless, an interesting diversity in their 
      expression, especially between individuals, was found.
FAU - Yin, H
AU  - Yin H
AD  - Department of Infectious Diseases and Clinical Microbiology, University of 
      Uppsala, Sweden.
FAU - Medstrand, P
AU  - Medstrand P
FAU - Andersson, M L
AU  - Andersson ML
FAU - Borg, A
AU  - Borg A
FAU - Olsson, H
AU  - Olsson H
FAU - Blomberg, J
AU  - Blomberg J
LA  - eng
SI  - GENBANK/U62098
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Breast/pathology/*virology
MH  - Breast Neoplasms/pathology/*virology
MH  - Cell Line
MH  - DNA, Complementary
MH  - DNA, Viral
MH  - Female
MH  - Gene Expression
MH  - Genome, Viral
MH  - Humans
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Placenta/pathology/*virology
MH  - Retroviridae/*genetics
MH  - Sequence Homology, Nucleic Acid
MH  - *Transcription, Genetic
EDAT- 1997/04/10 00:00
MHDA- 1997/04/10 00:01
CRDT- 1997/04/10 00:00
PHST- 1997/04/10 00:00 [pubmed]
PHST- 1997/04/10 00:01 [medline]
PHST- 1997/04/10 00:00 [entrez]
AID - 10.1089/aid.1997.13.507 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 1997 Apr 10;13(6):507-16. doi: 
      10.1089/aid.1997.13.507.