PMID- 9101091
OWN - NLM
STAT- MEDLINE
DCOM- 19970815
LR  - 20091119
IS  - 1044-9523 (Print)
IS  - 1044-9523 (Linking)
VI  - 8
IP  - 4
DP  - 1997 Apr
TI  - Inhibition of junction assembly in cultured epithelial cells by hepatocyte growth 
      factor/scatter factor is concomitant with increased stability and altered 
      phosphorylation of the soluble junctional molecules.
PG  - 451-62
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a mesenchymally derived 
      glycoprotein with a strong scattering effect on epithelial cells. A receptor 
      tyrosine kinase encoded by the met proto-oncogene has been identified as the 
      cellular receptor for HGF/SF. Following stimulation with HGF/SF, cell scattering 
      occurs concurrent with decreased cell-cell adhesion and disassembly of junctional 
      components. In culture, junction formation is cell-cell contact dependent and can 
      be regulated by modulating the Ca2+ concentrations of the growth media. 
      Decreasing the Ca2+ concentrations below 50 microM causes rapid disassembly of 
      junctions, whereas increasing the Ca2+ concentrations to 1.8 mM induces cell-cell 
      contact and junction assembly. Although associated with decreased cell-cell 
      adhesion and disassembly of the junctional complex, HGF/SF-induced scattering 
      occurs under high extracellular Ca2+ concentrations. To gain insight into the 
      mechanisms of HGF/SF-induced scattering of epithelial cells, we have studied the 
      effect(s) of HGF/SF on junction assembly by examining the solubility, stability, 
      phosphorylation, and subcellular localization of the major components of the 
      adhering junctions, plakoglobin (Pg) and E-cadherin, in Madin-Darby canine kidney 
      (MDCK) epithelial cells and in a MDCK cell line expressing an exogenous chimeric 
      met receptor (CSF-MET) that scatters in response to colony-stimulating factor 1 
      (CSF-1). The results have shown that in HGF/SF-stimulated MDCK cells, adhering 
      junctions were not assembled upon induction of cell-cell contact. 
      Immunofluorescence analyses showed that larger amounts of Pg and E-cadherin were 
      Triton X-100 extractable, and more significantly, these proteins were 
      homogeneously distributed along the membrane and were not concentrated at the 
      areas of cell-cell contact. Similar results were obtained for CSF-MET expressing 
      MDCK cells in response to CSF-1. In contrast, none of the above effects were 
      detected in MDCK cells expressing a mutant CSF-MET chimera containing a 
      phenylalanine substitution at tyrosine 1356 in met, which fails to scatter in 
      response to CSF-1. When compared with the unstimulated cells, the inhibition of 
      cell adhesion promoted by HGF/SF correlated with an increased stability of the 
      newly synthesized soluble E-cadherin and Pg and an altered phosphorylation 
      pattern of E-cadherin, as determined by partial proteolytic peptide mapping.
FAU - Pasdar, M
AU  - Pasdar M
AD  - Department of Anatomy and Cell Biology, University of Alberta, Edmonton, Canada. 
      mpasdar@anat.med.ualberta.ca
FAU - Li, Z
AU  - Li Z
FAU - Marreli, M
AU  - Marreli M
FAU - Nguyen, B T
AU  - Nguyen BT
FAU - Park, M
AU  - Park M
FAU - Wong, K
AU  - Wong K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Growth Differ
JT  - Cell growth & differentiation : the molecular biology journal of the American 
      Association for Cancer Research
JID - 9100024
RN  - 0 (Cadherins)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Colony-Stimulating Factors)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Desmoplakins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (gamma Catenin)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Animals
MH  - Cadherins/metabolism
MH  - Cell Adhesion/drug effects
MH  - Cell Adhesion Molecules/metabolism
MH  - Colony-Stimulating Factors/genetics/metabolism
MH  - Cytoskeletal Proteins/metabolism
MH  - Desmoplakins
MH  - Desmosomes/metabolism
MH  - Dogs
MH  - Epithelial Cells
MH  - Epithelium/drug effects
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Intercellular Junctions/*drug effects
MH  - Macrophage Colony-Stimulating Factor/metabolism
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins/genetics/metabolism
MH  - Proto-Oncogene Proteins c-met
MH  - Receptor Protein-Tyrosine Kinases/genetics/metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - gamma Catenin
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
PST - ppublish
SO  - Cell Growth Differ. 1997 Apr;8(4):451-62.