PMID- 9101478
OWN - NLM
STAT- MEDLINE
DCOM- 19970619
LR  - 20190516
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 59
IP  - 3
DP  - 1997 Mar
TI  - Course of apoptotic changes in the rat gastric mucosa caused by oral 
      administration of fusarenon-X.
PG  - 191-9
AB  - Male 5-week-old Wistar rats orally (po) administered with fusarenon-X (FX) 1.5 
      mg/kg and control rats po-treated with distilled water were sacrificed at 0-48 hr 
      after gavage. FX-administered rats showed significant dilatation of the stomach 
      with increased fluid contents at 1-24 hr postadministration (PA). 
      Histopathologically, karyopyknosis of chief cells in the basal region of the 
      gastric glands began to appear at 1 hr, and nuclear fragments were seen in the 
      neck cell region at 1.5 hr PA. At 2-4 hr PA, apoptotic cells appeared diffusely 
      in the neck region and focally in the basal region. Electron microscopy revealed 
      that cells phagocytosing apoptotic bodies were the surface epithelia, 
      undifferentiated neck cells, parietal cells and chief cells. No evidence was 
      detected to show that parietal cells underwent apoptotic changes. The apoptotic 
      lesions peaked at 4-6 hr PA, gradually subsided at 12 hr PA, and became minimal 
      leaving apoptotic remnants in the basal region at 24 hr PA. At 48 hr PA, however, 
      diffuse apoptotic lesions reappeared in the basal region at a level similar to 
      that at 2-3 hr PA. This might be attributable to absorption of FX retaining in 
      the stomach for 24 hr. In situ detection of DNA breaks by the terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) reaction was 
      consistent with the histopathologic findings. Agarose gel electrophoresis of DNA 
      fragments isolated from the gastric mucosae of FX-administered rats showed a 
      ladder pattern after 1.5 hr PA and the pattern became more distinct at 2-4 hr PA.
FAU - Li, J
AU  - Li J
AD  - Laboratory of Veterinary Pathology, Faculty of Agriculture, Kagoshima University, 
      Japan.
FAU - Shimizu, T
AU  - Shimizu T
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Mycotoxins)
RN  - 0 (Trichothecenes)
RN  - 23255-69-8 (fusarenon-X)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Apoptosis/*drug effects/physiology
MH  - Cell Nucleus/drug effects/ultrastructure
MH  - DNA/analysis
MH  - Electrophoresis, Agar Gel
MH  - Epithelium/chemistry/drug effects/physiology
MH  - Gastric Mucosa/*drug effects/*pathology/ultrastructure
MH  - Histocytochemistry/methods
MH  - Male
MH  - Microscopy, Electron
MH  - Mycotoxins/administration & dosage/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Time Factors
MH  - Trichothecenes/administration & dosage/*pharmacology
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - 10.1292/jvms.59.191 [doi]
PST - ppublish
SO  - J Vet Med Sci. 1997 Mar;59(3):191-9. doi: 10.1292/jvms.59.191.