PMID- 9103213
OWN - NLM
STAT- MEDLINE
DCOM- 19970612
LR  - 20190914
IS  - 1320-5463 (Print)
IS  - 1320-5463 (Linking)
VI  - 47
IP  - 4
DP  - 1997 Apr
TI  - Chromosomal translocations in human soft tissue sarcomas by interphase 
      fluorescence in situ hybridization.
PG  - 222-9
AB  - In soft tissue sarcomas, clonal rearrangement of chromosomes has been shown by 
      cytogenetic analysis to be unique and specific for tumor types. The development 
      of fluorescence in situ hybridization (FISH) has allowed detection of chromosomal 
      rearrangements in the interphase nuclei isolated from paraffin-embedded tissues. 
      Three kinds of translocations in the interphase nuclei that were isolated from 47 
      cases of soft tissue sarcomas were examined by FISH with chromosome-specific DNA 
      probes of centromeric and total probes. Of 47 soft tissue sarcomas 42 (89.4%) 
      revealed tumor-specific translocations by retrospective cytogenetic analysis. 
      Translocation t(X;18) was detected in 25/28 synovial sarcomas; translocation 
      t(11;22) in 5/6 Ewing's sarcomas and primitive neuroectodermal tumors (PNET); and 
      translocation t(12;16) was found in 12/13 liposarcomas, including 10 myxoid and 
      two round cell types as clonal chromosomal aberrations specific for both 
      subtypes. Based on the cytogenetic analysis, Ewing's sarcoma is related closely 
      with PNET as shown by MIC2-protein reactivity. Other cytogenetic findings of 
      translocation t(12;16) indicate that round cell liposarcomas share chromosomal 
      changes with myxoid liposarcomas, and further suggest that both tumor subtypes of 
      liposarcoma may possess common precursor cells. FISH is a useful aid in 
      determining the tumor type of soft tissue sarcomas with regard to histogenetic 
      origin.
FAU - Yoshida, H
AU  - Yoshida H
AD  - College of Medical Care Technology, Tottori University, Yonago, Japan.
FAU - Nagao, K
AU  - Nagao K
FAU - Ito, H
AU  - Ito H
FAU - Yamamoto, K
AU  - Yamamoto K
FAU - Ushigome, S
AU  - Ushigome S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Pathol Int
JT  - Pathology international
JID - 9431380
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - Chromosomes, Human, Pair 11
MH  - Chromosomes, Human, Pair 12
MH  - Chromosomes, Human, Pair 16
MH  - Chromosomes, Human, Pair 18
MH  - Chromosomes, Human, Pair 22
MH  - DNA Probes/chemistry
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Retrospective Studies
MH  - Sarcoma/*genetics/pathology
MH  - Soft Tissue Neoplasms/*genetics/pathology
MH  - *Translocation, Genetic
MH  - X Chromosome
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1111/j.1440-1827.1997.tb04484.x [doi]
PST - ppublish
SO  - Pathol Int. 1997 Apr;47(4):222-9. doi: 10.1111/j.1440-1827.1997.tb04484.x.