PMID- 9103241
OWN - NLM
STAT- MEDLINE
DCOM- 19970502
LR  - 20220227
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 61
IP  - 4
DP  - 1997 Apr
TI  - MCP-1-mediated chemotaxis requires activation of non-overlapping signal 
      transduction pathways.
PG  - 529-32
AB  - Monocyte chemoattractant protein-1 (MCP-1) is a CC chemokine that attracts 
      monocytes and T lymphocytes. Its receptor (CCR2) is a heptahelical 
      G-protein-coupled receptor (GPCR) whose signal transduction pathways for 
      chemotaxis have not been completely defined. Because other GPCRs stimulate the 
      mitogen-activated protein kinase (MAPK) cascade, we examined this pathway's 
      activity in response to MCP-1. MCP-1 induced rapid and transient activation of 
      MAPK in human monocytes and in Chinese hamster ovary cells expressing CCR2B. This 
      effect was largely insensitive to pertussis toxin and wortmannin, and was protein 
      kinase C-dependent and protein tyrosine kinase-independent. PD 098059, an 
      inhibitor of MEK activation, not only prevented MAPK activation but also 
      inhibited MCP-1-induced chemotaxis. Because pertussis toxin and wortmannin also 
      efficiently inhibit chemotaxis but do not completely inhibit MAPK activation, 
      these data may define non-overlapping signal transduction pathways that all must 
      be activated to produce chemokine-mediated chemotaxis.
FAU - Yen, H
AU  - Yen H
AD  - Division of Neoplastic Disease Mechanisms, Dana-Farber Cancer Institute, Harvard 
      Medical School, Boston, Massachusetts 02115, USA.
FAU - Zhang, Y
AU  - Zhang Y
FAU - Penfold, S
AU  - Penfold S
FAU - Rollins, B J
AU  - Rollins BJ
LA  - eng
GR  - CA53091/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (CCR2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Receptors, Cytokine)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
SB  - IM
MH  - Animals
MH  - CHO Cells/cytology/enzymology
MH  - Calcium-Calmodulin-Dependent Protein Kinases/metabolism
MH  - Chemokine CCL2/*physiology
MH  - Chemotaxis, Leukocyte/*physiology
MH  - Cricetinae
MH  - Enzyme Activation
MH  - Humans
MH  - Kinetics
MH  - Leukocytes, Mononuclear/cytology/enzymology
MH  - Mitogen-Activated Protein Kinase 1
MH  - Protein-Tyrosine Kinases/metabolism
MH  - Receptors, CCR2
MH  - *Receptors, Chemokine
MH  - Receptors, Cytokine/metabolism
MH  - Signal Transduction/*physiology
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1002/jlb.61.4.529 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1997 Apr;61(4):529-32. doi: 10.1002/jlb.61.4.529.