PMID- 9104557
OWN - NLM
STAT- MEDLINE
DCOM- 19970612
LR  - 20190831
IS  - 0425-1644 (Print)
IS  - 0425-1644 (Linking)
VI  - 29
IP  - 2
DP  - 1997 Mar
TI  - Nitric oxide production by equine articular cells in vitro.
PG  - 98-102
AB  - Recent research in several species has suggested nitric oxide (NO) as a mediator 
      of articular cartilage damage and an inhibitor of cartilage matrix neosynthesis. 
      This study investigated NO production by cultured equine articular chondrocytes 
      in response to 2 arthritogenic molecules, namely lipopolysaccharide (LPS) and 
      interleukin-1 beta (IL-1 beta), and compared NO production by cultured equine 
      synoviocytes stimulated with LPS. Synoviocytes exhibited a low basal level of NO 
      synthesis (measured as nitrite, a NO metabolite) that was neither significantly 
      increased nor decreased by exposure to LPS. Basal NO synthesis by synoviocytes 
      was not significantly reduced by competitive inhibitors of nitric oxide synthase 
      (NOS). In contrast, chondrocytes treated with LPS or IL-1 beta synthesised 
      nitrite in a dose-related manner. Inhibitors of NOS suppressed nitrite production 
      to below the basal levels of release of unstimulated cells. Dexamethasone, an 
      inhibitor of induction of the inducible isoform of NOS (iNOS), reduced nitrite 
      synthesis by LPS-stimulated chondrocytes. Western blot analysis revealed 
      expression, in response to LPS, of protein in the same molecular weight range as 
      iNOS identified in other species. This work demonstrates that equine chondrocytes 
      have the capacity to synthesise NO, although its exact roles in cartilage 
      metabolism have yet to be determined.
FAU - Frean, S P
AU  - Frean SP
AD  - Department of Veterinary Basic Sciences, Royal Veterinary College, North Mymms, 
      Herts, UK.
FAU - Bryant, C E
AU  - Bryant CE
FAU - Froling, I L
AU  - Froling IL
FAU - Elliott, J
AU  - Elliott J
FAU - Lees, P
AU  - Lees P
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Equine Vet J
JT  - Equine veterinary journal
JID - 0173320
RN  - 0 (Glucocorticoids)
RN  - 0 (Interleukin-1)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Nitrites)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
SB  - IM
CIN - Equine Vet J. 1997 Mar;29(2):86-7. PMID: 9104555
MH  - Animals
MH  - Blotting, Western/methods/veterinary
MH  - Cartilage, Articular/*cytology/drug effects/*metabolism
MH  - Cells, Cultured
MH  - Dexamethasone/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Glucocorticoids/pharmacology
MH  - Horses/*metabolism
MH  - Interleukin-1/pharmacology
MH  - Isomerism
MH  - Lipopolysaccharides/pharmacology
MH  - Nitric Oxide/*biosynthesis
MH  - Nitric Oxide Synthase/metabolism
MH  - Nitrites/metabolism
MH  - Synovial Membrane/cytology/drug effects/metabolism
MH  - Time Factors
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - 10.1111/j.2042-3306.1997.tb01649.x [doi]
PST - ppublish
SO  - Equine Vet J. 1997 Mar;29(2):98-102. doi: 10.1111/j.2042-3306.1997.tb01649.x.