PMID- 9105406
OWN - NLM
STAT- MEDLINE
DCOM- 19970501
LR  - 20190623
IS  - 0006-2952 (Print)
IS  - 0006-2952 (Linking)
VI  - 53
IP  - 4
DP  - 1997 Feb 21
TI  - Expression of endothelin-1 in human broncho-epithelial and monocytic cell lines: 
      influence of tumor necrosis factor-alpha and dexamethasone.
PG  - 547-52
AB  - Abnormal endothelin-1 (ET-1) expression has been observed in bronchial asthma and 
      systemic sclerosis with lung involvement. The purpose of this study was to 
      analyze the synthesis of ET-1 in human airway epithelial cells and macrophages 
      under basal conditions and after challenge with tumor necrosis factor-alpha (TNF 
      alpha) or with the glucocorticoid dexamethasone. The ET-1 mRNA level and peptide 
      release were measured in the broncho-epithelial cells BEAS-2B and the monocytic 
      cell line U937. At baseline, U937 cells released low amounts of ET-1 peptide, 
      whereas ET-1 was not detectable in BEAS-2B cells. After TNF alpha treatment, 
      BEAS-2B cells, but not U937 cells, showed a significant increase in ET-1 
      expression, both at the mRNA and peptide levels. In contrast, dexamethasone 
      elicited an increased amount of ET-1 peptide in U937 medium, but not in BEAS-2B 
      cells. In this latter cell line, dexamethasone pretreatment was unable to inhibit 
      the TNF alpha-induced expression. We conclude that response to TNF alpha and 
      glucocorticoids is cell-type specific with respect to ET-1 production. The 
      response of lung tissue to these agents in vivo is likely to be the overall 
      balance of induction and inhibition in local microenvironments.
FAU - Aubert, J D
AU  - Aubert JD
AD  - Department de Medicine, BH-07 CHUV, CH-1011 Lausanne, Switzerland. 
      john-David.Aubert@chuv.hospvd.ch
FAU - Juillerat-Jeanneret, L
AU  - Juillerat-Jeanneret L
FAU - Leuenberger, P
AU  - Leuenberger P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem Pharmacol
JT  - Biochemical pharmacology
JID - 0101032
RN  - 0 (Endothelin-1)
RN  - 0 (Endothelins)
RN  - 0 (Protein Precursors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
EIN - Biochem Pharmacol 1997 Jun 15;53(12):1945
MH  - Blotting, Northern
MH  - Bronchi/*metabolism
MH  - Cell Line
MH  - Dexamethasone/*pharmacology
MH  - Endothelin-1/*biosynthesis
MH  - Endothelins/genetics
MH  - Humans
MH  - Monocytes/*metabolism
MH  - Protein Precursors/genetics
MH  - RNA, Messenger/analysis
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1997/02/21 00:00
MHDA- 1997/02/21 00:01
CRDT- 1997/02/21 00:00
PHST- 1997/02/21 00:00 [pubmed]
PHST- 1997/02/21 00:01 [medline]
PHST- 1997/02/21 00:00 [entrez]
AID - S000629529600771X [pii]
AID - 10.1016/s0006-2952(96)00771-x [doi]
PST - ppublish
SO  - Biochem Pharmacol. 1997 Feb 21;53(4):547-52. doi: 10.1016/s0006-2952(96)00771-x.