PMID- 9106509
OWN - NLM
STAT- MEDLINE
DCOM- 19970512
LR  - 20220408
IS  - 0167-4412 (Print)
IS  - 0167-4412 (Linking)
VI  - 33
IP  - 5
DP  - 1997 Mar
TI  - Characterization of the gene for delta1-pyrroline-5-carboxylate synthetase and 
      correlation between the expression of the gene and salt tolerance in Oryza sativa 
      L.
PG  - 857-65
AB  - A cDNA for delta1-pyrroline-5-carboxylate (P5C) synthetase (cOsP5CS), an enzyme 
      involved in the biosynthesis of proline, was isolated and characterized from a 
      cDNA library prepared from 14-day-old seedlings of Oryza sativa cv. Akibare. The 
      deduced amino acid sequence of the P5CS protein (OsP5CS) from O. sativa exhibited 
      74.2% and 75.5% homology to that of the P5CS from Arabidopsis thaliana and Vigna 
      aconitifolia, respectively. Northern blot analysis revealed that the gene for 
      P5CS (OsP5CS) was induced by high salt, dehydration, treatment of ABA and cold 
      treatment, while it was not induced by heat treatment. Simultaneously, 
      accumulation of proline was observed as a result of high salt treatment in O. 
      sativa. Moreover, the levels of expression of OsP5CS mRNA and content of proline 
      under salt stress condition were compared between a salt-tolerant cultivar, 
      Dee-gee-woo-gen (DGWG) and a salt-sensitive breeding line, IR28. It was observed 
      that the expression of the P5CS gene and the accumulation of proline in DGWG 
      steadily increased, whereas those in IR28 increased slightly.
FAU - Igarashi, Y
AU  - Igarashi Y
AD  - Advanced Research Laboratory, Hitachi Ltd., Hatoyama, Saitama, Japan.
FAU - Yoshiba, Y
AU  - Yoshiba Y
FAU - Sanada, Y
AU  - Sanada Y
FAU - Yamaguchi-Shinozaki, K
AU  - Yamaguchi-Shinozaki K
FAU - Wada, K
AU  - Wada K
FAU - Shinozaki, K
AU  - Shinozaki K
LA  - eng
SI  - GENBANK/D49714
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Plant Mol Biol
JT  - Plant molecular biology
JID - 9106343
RN  - 0 (DNA, Complementary)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 9DLQ4CIU6V (Proline)
RN  - EC 1.2.1.88 (1-Pyrroline-5-Carboxylate Dehydrogenase)
RN  - EC 1.5.- (Oxidoreductases Acting on CH-NH Group Donors)
SB  - IM
MH  - 1-Pyrroline-5-Carboxylate Dehydrogenase
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Drug Resistance
MH  - Gene Expression
MH  - *Genes, Plant
MH  - Molecular Sequence Data
MH  - Oryza/drug effects/enzymology/*genetics
MH  - Osmotic Pressure
MH  - Oxidoreductases Acting on CH-NH Group Donors/*genetics
MH  - Proline/*biosynthesis
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Sodium Chloride/*pharmacology
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - 10.1023/a:1005702408601 [doi]
PST - ppublish
SO  - Plant Mol Biol. 1997 Mar;33(5):857-65. doi: 10.1023/a:1005702408601.